Polyplex gene delivery modulated by redox potential gradients
Polyplex gene delivery modulated by redox potential gradients
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DOI：
10.1080/10611860600834409
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发表时间：
2006-09-01
影响因子：
4.5
通讯作者：
Oupicky, David
中科院分区：
文献类型：
--
作者：
Manickam, Devika Soundara;Oupicky, David
对氧化还原电位梯度敏感的多聚体是一类很有前途的核酸载体。本文就这些载体的研究进展作一综述。讨论了设计氧化还原敏感多聚物的生物学原理，以及在多聚物结构中引入可还原二硫键的基本合成方法。本文综述了质粒DNA、mRNA、反义寡核苷酸和siRNA的氧化还原敏感复合物的生物学特性，重点介绍了< i >在体外细胞传递、细胞毒性和< i >在体内的活性。总的来说，氧化还原敏感的多聚体代表了一个有前途的平台，可以进一步发展为多种治疗性核酸的载体。
< p > Polyplexes sensitive to redox potential gradients represent a promising class of vectors for delivery of nucleic acids. This review focuses on the recent advances in the development of these vectors. The biological rationale for the design of redox-sensitive polyplexes is discussed together with the basic synthetic approaches for introducing reducible disulfide bonds into the structure of the polyplexes. The biological properties of the redox-sensitive polyplexes of plasmid DNA, mRNA, antisense oligonucleotides and siRNA are reviewed with emphasis on < i > in vitro cellular delivery, cytotoxicity and < i > in vivo activity. Overall, redox-sensitive polyplexes represent a promising platform for further development as vectors for delivery of a wide variety of therapeutic nucleic acids.