Early diagnostic potential for hepatocellular carcinoma using the SELDI ProteinChip system
Early diagnostic potential for hepatocellular carcinoma using the SELDI ProteinChip system
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DOI：
10.1002/hep.21598
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发表时间：
2007-04-01
期刊：
影响因子：
13.5
通讯作者：
Tsubouchi, Hirohito
中科院分区：
文献类型：
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作者：
Kanmura, Shuji;Uto, Hirofumi;Tsubouchi, Hirohito
肝细胞癌的早期发现增加了根治治疗的可能性，提高了存活率。为了促进肝细胞癌的早期发现，本研究试图利用表面增强激光解吸电离飞行时间质谱仪(SELDlTOF/MS)蛋白质芯片技术寻找新的肝细胞癌诊断标志物。血清样本来自153名患有或不患有肝癌的患者，他们都被诊断出患有丙型肝炎相关的慢性肝病。用SELDI-TOF/MS对血清标本进行分析，以确定与肝细胞癌相关的蛋白质。我们利用35例肝细胞癌患者和44例非肝细胞癌患者的血清样本构建了一棵初步的决策树，用于正确诊断肝细胞癌。选择6个蛋白质峰来构建决策树，使用第一组。然后，使用第二组患者(n=29)和非肝癌患者(n=33)来评估决策树的有效性。该决策树诊断肝癌的敏感度和特异度分别为830/6和76%。对于第三组，我们分析了7例经超声诊断为肝细胞癌(US)前的肝细胞癌患者和5例过去3年未患肝细胞癌的患者的血清。这些诊断标记物的使用预测了这7名患者中的6名患者在肝细胞癌临床表现明显之前诊断为肝细胞癌，没有任何假阳性。结论：应用DIE-SELDI蛋白质芯片系统检测血清标本有助于早期发现和预测慢性丙型肝炎患者肝细胞癌的发生。
Early detection of HCC increases the potential for curative treatment and improves survival. To facilitate early detection of HCC, this study sought to identify novel diagnostic markers of HCC using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDlTOF/MS) ProteinChip technology. Serum samples were obtained from 153 patients with or without HCC, all of whom had been diagnosed with HCV-associated chronic liver disease. To identify proteins associated with HCC, serum samples were analyzed using SELDI-TOF/MS. We constructed an initial decision tree for the correct diagnosis of HCC using serum samples from patients with (n := 35) and without (n = 44) HCC. Six protein peaks were selected to construct a decision tree using this first group. The efficacy of the decision tree was then assessed using a second group of patients with (n = 29) and without (n = 33) HCC. The sensitivity and specificity of this decision tree for the diagnosis of HCC were 830/6 and 76%, respectively. For a third group, we analyzed sera from seven patients with HCC obtained before the diagnosis of HCC by ultrasonography (US) and from five patients free of HCC for the past 3 years. Use of these diagnostic markers predicted the diagnosis of HCC in six of these seven patients before HCC was clinically apparent without any false positives. Conclusion: Serum profiling using die SELDI ProteinChip system is useful for the early detection and prediction of HCC in patients with chronic HCV infeiction.