Collagen XVIII Deposition in the Basement Membrane Zone beneath the Newly Forming Epidermis during Wound Healing in Mice
Collagen XVIII Deposition in the Basement Membrane Zone beneath the Newly Forming Epidermis during Wound Healing in Mice
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DOI：
10.18926/amo/56649
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发表时间：
2019-04-01
影响因子：
0.5
通讯作者：
Oohashi, Toshitaka
中科院分区：
文献类型：
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作者：
Maeba, Takahiro;Yonezawa, Tomoko;Oohashi, Toshitaka
基底膜（BM）由多种细胞外分子组成，并调节组织再生和维持。在这里，我们证明了在小鼠切除伤口夹板模型中，皮肤伤口愈合期间，胶原蛋白XVIII在BM中时空表达。在伤后第3天和第6天检测到上皮再生。在第3天，表皮BM的超微结构是不连续的，而在第6天，在伤口边缘的近端区域观察到连续的BM。免疫组化显示，胶原蛋白XVIII沉积在BM区下新形成的表皮在第3和6天的伤口。层粘连蛋白-332，已知是最早出现在伤口中的BM组分，在第3天和第6天在表皮BM区与胶原XVIII共定位。α 1（IV）胶原和巢蛋白-1在表皮BM区的沉积发生晚于胶原XVIII。我们还观察到短同种型的胶原蛋白XVIII在表皮BM区在第3天受伤后。总的来说，我们的研究结果表明，胶原蛋白XVIII在上皮再形成过程中真皮-表皮连接的形成中起作用，并且它是参与上皮再形成早期阶段的短同种型。
The basement membrane (BM) is composed of various extracellular molecules and regulates tissue regeneration and maintenance. Here, we demonstrate that collagen XVIII was spatiotemporally expressed in the BM during skin wound healing in a mouse excisional wound-splinting model. Re-epithelialization was detected at days 3 and 6 post-wounding. The ultrastructure of epidermal BM was discontinuous at day 3, whereas on day 6 a continuous BM was observed in the region proximal to the wound edge. Immunohistochemistry demonstrated that collagen XVIII was deposited in the BM zone beneath newly forming epidermis in day 3 and 6 wounds. Laminin-332, known to be the earliest BM component appearing in wounds, was colocalized with collagen XVIII in the epidermal BM zone at days 3 and 6. The deposition of alpha 1(IV) collagen and nidogen-1 in the epidermal BM zone occurred later than that of collagen XVIII. We also observed the short isoform of collagen XVIII in the epidermal BM zone at day 3 post-wounding. Collectively, our results suggested that collagen XVIII plays a role in the formation of the dermal-epidermal junction during re-epithelialization, and that it is the short isoform that is involved in the early phase of re-epithelialization.