Label-free reading of microarray-based proteins with high throughput surface plasmon resonance imaging
Label-free reading of microarray-based proteins with high throughput surface plasmon resonance imaging
复制标题
通过高通量表面等离子共振成像对基于微阵列的蛋白质进行无标记读取
DOI：
10.1016/j.bios.2006.01.025
复制
发表时间：
2006-12-15
影响因子：
12.6
通讯作者：
Chen, Yi
中科院分区：
文献类型：
--
作者：
Huang, Haowen;Chen, Yi
利用表面等离子体共振（SPR）成像技术，提出了一种在金表面识别蛋白质的简单方法。作为一种高通量的方法，牛血清白蛋白（BSA），聚-L-赖氨酸（PL），酪蛋白和乳酸脱氢酶（LDG）的蛋白质阵列的制造和SPR成像能够检测从不同种类的蛋白质固定在传感器表面。这些蛋白质可以通过不同的反射强度或改变光的入射角位置来直接区分。用变性剂6 M GdnHCl溶液对固定化蛋白质进行变性处理，发现变性后SPR传感器的反射强度发生了明显的变化。这些蛋白质变性的观察进一步支持了在变性前蛋白质阵列上可以区分不同蛋白质的事实。另一方面，变性过程提供了有用的信息，即随着变性过程的进行，分子结构的任何变化都会导致SPR信号的变化。实现了芯片到芯片的良好再现性，用于无标记区分各种蛋白质。(c)2006 Elsevier B. V.保留所有权利。
A simple method is presented discriminating proteins at a gold surface by using an emerging technology, surface plasmon resonance (SPR) imaging. As a high throughput method, the protein array of bovine serum albumin (BSA), poly-L-lysine (PL), casein and lactate dehydrogenase (LDG) was fabricated and SPR imaging enables detection from different kinds of proteins immobilized on the sensor surface. These proteins can be discriminated directly by various reflected intensity or changing the incident angular position of light. Denaturation of these immobilized proteins on SPR sensor by interacting with denaturant 6 M GdnHCl solution was also performed and obvious changes in reflected intensity were occurred after denaturation. The observation of denaturation of these proteins further supported the fact that different proteins could be discriminated on protein array before denaturation. On the other hand, the procedure of denaturation provided useful information that any change of molecular structure with the progress of denaturation would result in change of SPR signal. Excellent reproducibility with a chip-to-chip for label-free discriminating various proteins was achieved. (c) 2006 Elsevier B.V. All rights reserved.