An E3-like factor that promotes SUMO conjugation to the yeast septins
An E3-like factor that promotes SUMO conjugation to the yeast septins
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DOI：
10.1016/s0092-8674(01)00491-3
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发表时间：
2001-09-21
期刊：
影响因子：
64.5
通讯作者：
Gupta, AA
中科院分区：
文献类型：
--
作者：
Johnson, ES;Gupta, AA
泛素相关蛋白SUMO与其他蛋白质的共价连接参与了许多过程，包括信号转导，转录调控和生长控制。我们报告Siz1作为SUMO途径中的E3样因子的表征。SUMO连接到S需要尺寸1。cerevisiae septins在体内并强烈刺激septin在体外的sumoylation。Siz1和相关蛋白Siz2在细胞周期的不同阶段促进SUMO与不同底物的结合，并且一起是酵母中大多数SUMO结合所需的。Siz 1、Siz 2和皮亚斯（活化STAT的蛋白质抑制剂）蛋白形成由不寻常的RING相关基序定义的保守家族。我们的研究结果表明，这个家庭的功能，促进SUMO共轭特定底物。
Covalent attachment of the ubiquitin-related protein SUMO to other proteins participates in many processes including signal transduction, transcriptional regulation, and growth control. We report the characterization of Siz1 as an E3-like factor in the SUMO pathway. Siz1 is required for SUMO attachment to the S. cerevisiae septins in vivo and strongly stimulates septin sumoylation in vitro. Siz1 and the related protein Siz2 promote SUMO conjugation to different substrates at different stages of the cell cycle and, together, are required for most SUMO conjugation in yeast. Siz1, Siz2, and the PIAS (protein inhibitor of activated STAT) proteins form a conserved family defined by an unusual RING-related motif. Our results suggest that this family functions by promoting SUMO conjugation to specific substrates.