Autonomous modes of behavior in primordial germ cell migration
Autonomous modes of behavior in primordial germ cell migration
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DOI：
10.1016/s1534-5807(04)00074-7
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发表时间：
2004-04-01
影响因子：
11.8
通讯作者：
Raz, E
中科院分区：
文献类型：
--
作者：
Reichman-Fried, M;Minina, S;Raz, E
斑马鱼原始生殖细胞（PGCs）被趋化因子SDF-1a引导向它们的靶点。PGCs在其迁移的三个阶段：当迁移作为单个细胞，同时保持在一个集群的配置，当移动作为一个细胞簇在胚胎内。我们发现，个别迁移PGCs迁移和暂停模式之间的交替。暂停间隔的特征在于细胞极性的丧失，并与随后的迁移方向的变化相关。这些属性构成了PGC的固有行为，使得能够擦除先前的极性并对环境进行重新采样。在高SDF-1a水平的地点迁移停止后，PGCs作为集群恢复迁移。看似协调的集群迁移是响应SDF-1a分布的局部变化的单细胞移动的结果。总之，这些行为模式允许细胞以高保真度到达特定目的地并保持在其目标位置。
Zebrafish primordial germ cells (PGCs) are guided toward their targets by the chemokine SDF-1a. PGCs were followed during three phases of their migration: when migrating as individual cells, while remaining in a clustered configuration, and when moving as a cell cluster within the embryo. We found that individually migrating PGCs alternate between migratory and pausing modes. Pausing intervals are characterized by loss of cell polarity and correlate with subsequent changes in the direction of migration. These properties constitute an intrinsic behavior of PGCs, enabling erasure of prior polarity and re-sampling of the environment. Following migration arrest at a site of high SDF-1a levels, PGCs resume migration as a cluster. The seemingly coordinated cluster migration is a result of single-cell movement in response to local variations in SDF-1a distribution. Together, these behavioral modes allow the cells to arrive at specific destinations with high fidelity and remain at their target site.