Characterization of a cDNA clone encoding a Brassica napus 12 S protein (cruciferin) subunit. Relationship between precursors and mature chains.
Characterization of a cDNA clone encoding a Brassica napus 12 S protein (cruciferin) subunit. Relationship between precursors and mature chains.
复制标题
编码甘蓝型油菜 12 S 蛋白（十字花科素）亚基的 cDNA 克隆的表征。
DOI：
10.1016/s0021-9258(19)39861-8
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
L. Rask
中科院分区：
文献类型：
--
作者：
J. Rödin;M. Ericson;L. Josefsson;L. Rask
十字花科素（12 S 球蛋白）是一种大的中性寡聚蛋白，在油菜籽（Brassica napus）种子发育过程中合成。它是主要的种子蛋白，由六个亚基对组成。这些对中的每一对都是作为包含一条重α链和一条轻β链的前体合成的。十字花科素的电泳分析表明存在四种不同的α链和四种不同的β链。从胚胎 cDNA 文库中选择十字花科素克隆。该克隆 pCRU1 包含一个 1518 个碱基对的开放阅读框，对应于截短的 NH2 末端信号序列，后跟 296 个氨基酸残基的 α 链和 190 个氨基酸残基的 β 链。对各个十字花科素链及其肽进行NH 2 末端氨基酸序列分析。从特定的 α 链和 β 链对（α 1 和 β 1）获得的序列与 pCRU1 推导的氨基酸序列完全一致。进一步的比较表明，先前鉴定的十字花科素 cDNA 克隆编码密切相关的 α 2/α 3-β 2/β 3 亚基的前体之一。推导的两个 cDNA 克隆的氨基酸序列显示出 64% 的相似性。
Cruciferin (12 S globulin) is a large, neutral, oligometric protein synthesized in rapeseed (Brassica napus) during seed development. It is the major seed protein and is composed of six subunit pairs. Each of these pairs is synthesized as a precursor containing one heavy alpha-chain and one light beta-chain. Electrophoretic analysis of cruciferin showed that four different alpha- and four different beta-chains exist. A cruciferin clone was selected from an embryo cDNA library. This clone, pCRU1, contains a 1518-base pair open reading frame corresponding to a truncated NH2-terminal signal sequence followed by an alpha-chain of 296 and a beta-chain of 190 amino acid residues. Individual cruciferin chains as well as peptides thereof were subjected to NH2-terminal amino acid sequence analysis. The sequences obtained from a specific alpha- and beta-chain pair (alpha 1 and beta 1) showed total identity with the deduced amino acid sequence from pCRU1. Further comparisons revealed that a previously characterized cruciferin cDNA clone encodes one of the precursors for the closely related alpha 2/ alpha 3-beta 2/beta 3 subunits. The deduced amino acid sequences of the two cDNA clones display 64% similarity.