Characterisation of insoluble a-1,3-/a-1,6 mixed linkage glucan produced in addition to soluble a-1,6-linked dextran by glucansucrase (DEX-N) from Leuconostoc citreum ABK-1.
Characterisation of insoluble a-1,3-/a-1,6 mixed linkage glucan produced in addition to soluble a-1,6-linked dextran by glucansucrase (DEX-N) from Leuconostoc citreum ABK-1.
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除了可溶性 a-1,6-连接葡聚糖之外，还通过柠檬明串珠菌 ABK-1 的葡聚糖蔗糖酶 (DEX-N) 产生的不溶性 a-1,3-/a-1,6 混合连接葡聚糖的表征。
DOI：
10.1016/j.ijbiomac.2020.02.247
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发表时间：
2020
影响因子：
8.2
通讯作者：
Wangpaiboon K
中科院分区：
文献类型：
--
作者：
Wangpaiboon K
葡聚糖酶催化蔗糖生成葡聚糖。成功克隆了leuconostoccitreumabk -1葡聚糖酶编码基因dex-N，表达了inE。coliBL21星（DE3）。DEX-N产生两种类型的葡聚糖：可溶性（s -葡聚糖）和不溶性（i -葡聚糖）葡聚糖。s -葡聚糖的大小约为10 kDa，含有约90%的α-1,6键；就其水溶性而言，这与商业右旋糖酐相似。另一方面，i -葡聚糖不溶于水，在其结构中具有α-1,3和α-1,6键的嵌段模式。值得注意的是，i -葡聚糖的FTIR和粉末x射线衍射图显示了α-1,6-链葡聚糖和α-1,3-链突变体的结合特征。虽然i -葡聚糖和变形聚糖都是不溶性葡聚糖，但它们的物理特性明显不同。
Glucansucrases catalyse the formation of glucans from sucrose. The glucansucrase-encoding gene fromLeuconostoccitreumABK-1,dex-N, was successfully cloned and expressed inE. coliBL21 Star (DE3). DEX-N produces 2 types of glucans: soluble (S-dextran) and insoluble (I-glucan) glucans. The S-dextran was determined to be ca. 10 kDa in size and contained >90% α-1,6 linkages; along with its water solubility, this is similar to commercial dextran. On the other hand, I-glucan was water-insoluble, harbouring a block-wise pattern of α-1,3 and α-1,6 linkages in its structure. Notably, the FTIR and powder X-ray diffraction pattern of I-glucan exhibited a combination of features found in α-1,6-linked dextran and α-1,3-linked mutan. Although both I-glucan and mutan are insoluble glucans, their physical characteristics are notably dissimilar.