Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains.
Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains.
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两种与变性小鼠 II 类 β 链反应的新型小鼠单克隆抗体的制备和表征。
DOI：
10.1089/hyb.1992.11.217
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Frelinger,JA
中科院分区：
文献类型：
--
作者：
LaPan,KE;Klapper,DG;Frelinger,JA
我们报告的生产和表征的两个鼠肽特异性抗主要组织相容性复合物II类链特异性单克隆抗体。制备了两种新的鼠源单克隆抗体，它们分别与AB氨基酸（146-157）和AB氨基酸（146-157）的合成肽反应。KL 295是小鼠抗Ab单克隆抗体，其与H-2b、H-2d、H-2 P和H-2 q的变性β链反应，但不与H-2f、H-2 j、H-2k和H-2S小鼠的脾细胞裂解物反应。相反，小鼠抗Ab单克隆抗体KL 304与H-2f、H-2 j、H-2k和H-2S小鼠的变性II类β链反应，但不与H-2b结合，H-2d、H-2 P或H-2 q β链，表明该分子的残基153-155对表位至关重要。KL 295和KL 304均结合B10.WB（H-2 j），这表明该品系小鼠中存在独特的表位。KL 295或KL 304均不与天然小鼠II类细胞表面分子反应。
We report on the production and characterization of two murine peptide specific anti-major histocompatibility complex class II chain specific monoclonal antibodies. Two new mouse monoclonal antibodies reactive with two synthetic peptides corresponding to Abbamino acids (146-157) and Absamino acids(146-157) were produced. KL295 is the mouse anti-Abbmonoclonal antibody, which reacts with denatured β chains of H-2b, H-2d, H-2P, and H-2q, butfail to react with spleen cell lysates from H-2f, H-2j, H-2kand H-2Smice The mouse anti Absmonoclonal antibody, KL304 in contrast reacts with the denatured Class II β chains of H-2f, H-2j, H-2kand H-2Smice, but fails to bind H-2b, H-2d, H-2Por H-2qβ chain, suggesting residues 153-155 of this molecule to be critical for the epitope. Both KL295 and KL304 bind B10.WB (H-2j) which suggests a unique epitope in this strain of mice. Neither KL295 or KL304 react with native mouse class II cell surface molecules.