Multi-spectrometric analyses of lipoteichoic acids isolated from Lactobacillus plantarum
Multi-spectrometric analyses of lipoteichoic acids isolated from Lactobacillus plantarum
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DOI：
10.1016/j.bbrc.2011.03.107
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发表时间：
2011-04-22
影响因子：
3.1
通讯作者：
Kim, Byung-Gee
中科院分区：
文献类型：
--
作者：
Jang, Kyoung-Soon;Baik, Jung Eun;Kim, Byung-Gee
脂磷壁酸是革兰氏阳性菌的主要细胞壁毒力因子。来自各种细菌的LTA由于其结构的异质性而具有不同的免疫刺激潜力。尽管最近的研究已经证明从植物乳杆菌（Lactobacillus plantarum，pLTA）中分离的LTA具有抗炎特性，并且比来自病原菌的LTA的炎症性更小，但对pLTA的结构知之甚少。在这项研究中，pLTA的高场NMR谱进行了比较，从致病菌，金黄色葡萄球菌（阿尔塔）的LTA。2D NMR结果表明pLTA在聚甘油磷酸骨架上具有α-连接的己糖取代基而不是N-乙酰基-葡糖胺取代基，并且其糖脂中具有不饱和脂肪酸。通过HPAEC-PAD分析，糖取代基显示为葡萄糖与半乳糖的约29：1摩尔比。MALDI-TOF/TOF MS分析鉴定了pLTA的糖脂部分中不饱和脂肪酸的存在。此外，发现糖脂结构由三己糖基-二酰基-和/或三己糖基-三酰基-甘油神经酰胺单元通过糖脂的独特碎片离子组成。这些结果使我们能够阐明pLTA的结构，这是明显不同于典型的LTA结构，并建议pLTA的独特的免疫学性质可能是由pLTA的结构。(C)2011 Elsevier Inc. All rights reserved.
Lipoteichoic acid is a major cell wall virulence factor of gram-positive bacteria. LTAs from various bacteria have differential immunostimulatory potentials due to heterogeneity in their structures. Although recent studies have demonstrated that LTA isolated from Lactobacillus plantarum (pLTA) has anti-inflammatory properties and is less inflammatory than LTAs from pathogenic bacteria, little is known about the structure of pLTA. In this study, high-field NMR spectra of the pLTA were compared with those of LTA from pathogenic bacterium, Staphylococcus aureus (aLTA). The 2D NMR results demonstrated that pLTA possesses alpha-linked hexose sugar substituents on the poly-glycerophosphate backbone instead of N-acetyl-glucosamine substituents, and unsaturated fatty acids in its glycolipids. The sugar substituents were revealed as an approximately 29:1 molar ratio of the glucose to galactose by HPAEC-PAD analysis. MALDI-TOF/TOF MS analyses identified the presence of unsaturated fatty acids in the glycolipid moieties of pLTA. In addition, the glycolipid structure was found to be composed of trihexosyl-diacyl- and/or trihexosyl-triacyl-glycerol ceramide units by means of unique fragment ions of the glycolipids. These results enabled us to elucidate the pLTA structure, which is distinctively different from canonical LTA structure, and suggest that the unique immunological property of pLTA might be caused by the pLTA structure. (C) 2011 Elsevier Inc. All rights reserved.