A reconstituted cell-free system for the specific transfer of steroid--receptor complexes into nuclear chromatin isolated from the rat ventral prostate gland.
A reconstituted cell-free system for the specific transfer of steroid--receptor complexes into nuclear chromatin isolated from the rat ventral prostate gland.
复制标题
一种重建的无细胞系统，用于将类固醇受体复合物特异性转移到从大鼠腹侧前列腺分离的核染色质中。
作者：
W. Mainwaring;B. Peterken
1. A system has been developed for the specific transfer of [(3)H]dihydrotestosterone-receptor complexes into prostatic chromatin in vitro. 2. Under optimum conditions the overall transfer of [(3)H]dihydrotestosterone into purified chromatin in this reconstituted system is entirely consistent with the results obtained in whole tissue both in vivo and in vitro. 3. The transfer of [(3)H]dihydrotestosterone into chromatin is tissue-specific and maximal into chromatin isolated from androgen-dependent tissues. 4. The tissue specificity is maintained at two levels: first, in the presence of specific cytoplasmic androgen-receptor proteins; secondly, by the nature and composition of the chromatin itself. 5. Evidence is presented that androgenic steroids in vivo may maintain the tissue-specific nature of chromatin in androgen-dependent tissues by the selective induction of nuclear protein synthesis. 6. The relevance of these findings to the mechanism of action of androgenic steroids is discussed.
