Identification of proteins downregulated during the postnatal development of the cat visual cortex.
Identification of proteins downregulated during the postnatal development of the cat visual cortex.
复制标题
鉴定猫视觉皮层出生后发育过程中下调的蛋白质。
DOI：
10.1093/cercor/4.4.361
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Hockfield,S
中科院分区：
文献类型：
--
作者：
Kind,P;Blakemore,C;Fryer,H;Hockfield,S
为了确定在哺乳动物视觉皮层的发展中发挥作用的蛋白质，我们已经使用了免疫抑制和快速免疫策略，以产生单克隆抗体的抗原存在于17区的猫在皮质可塑性的高峰期，但下调接近年底的塑料期。我们在这里报告的免疫组织化学和免疫生物化学表征的6种单克隆抗体，识别抗原优先表达在猫的视觉皮层在5周龄。单克隆抗体Cat-305和Cat-306检测三个免疫反应性元素，出生时不存在，但在5周。大多数免疫反应性与15周龄时缺失的白色物质中的细胞群相关。在5周和15周时，非常少量的神经元在所有过程中都表现出强烈的免疫反应性，类似于用高尔基体染色所实现的。此外，IV层中的免疫反应性弥散带主要局限于皮质区17和18。Cat-307识别存在于小细胞质内含物中的150 kDa可溶性蛋白。这些细胞质“点”存在于所有层中，但在第V层中最突出。Cat-307免疫反应性在出生时存在，并在15周时完全下调。Cat-104和Cat-105识别出生时和5周时存在的200 kDa不溶性蛋白，但在15周时显著下调。出生时，白色物质、基板和第I层标记最密集，而5周龄时，第II、III和V层标记不明显。Cat-402识别许多在5周龄和15周龄时差异表达的高分子量抗原。在5周和15周时，所有层中都存在类似于原生质星形胶质细胞的染色非神经元细胞。在5周，但不是在出生时或15周，黑暗的免疫反应放射状的过程中观察到，贯穿整个深度的cortex.We在这里显示，免疫反应的几种不同的单克隆抗体检测选择性期间的最大发育可塑性。结果表明，猫5周龄时的视觉皮层与15周龄时的皮层在分子上是不同的。
To identify proteins that play a role in the development of the mammalian visual cortex, we have used an immunosuppression and rapid immunization strategy to generate monoclonal antibodies to antigens that are present in area 17 of the cat during the peak of cortical plasticity but are downregulated near the end of the plastic period. We report here the immunohistochemical and immunobiochemical characterization of six monoclonal antibodies that identify antigens preferentially expressed in the cat visual cortex at 5 weeks of age.Monoclonal antibodies Cat-305 and Cat-306 detect three immunoreactive elements that are not present at birth but are present at 5 weeks. The majority of immunoreactivity is associated with a population of cells in the white matter that are absent at 15 weeks of age. At both 5 and 15 weeks, a very small number of neurons show intense immunoreactivity throughout all processes, resembling that achieved with a Golgi stain. In addition, a diffuse band of immunoreactivity in layer IV is largely restricted to cortical areas 17 and 18.Cat-307 recognizes a 150 kDa soluble protein present in small cytoplasmic inclusions. These cytoplasmic “dots” are present in all layers, but are most prominent in layer V. Cat-307 immunoreactivity is present at birth and is completely downregulated by 15 weeks. Cat-104 and Cat-105 recognize a 200 kDa insoluble protein present at birth and at 5 weeks, but markedly downregulated by 15 weeks. At birth, the white matter, subplate, and layer I are most densely labeled, while at 5 weeks labeling is densest in layers II, III, and V. Cat-402 recognizes a number of high-molecular-weight antigens that are differentially expressed at 5 and 15 weeks of age. Stained non-neuronal cells that resemble protoplasmic astrocytes are present in all layers at both 5 and 15 weeks. At 5 weeks, but not at birth or 15 weeks, darkly immunoreactive radial processes are observed that run through the full depth of the cortex.We show here that immunoreactivity for several different monoclonal antibodies is detected selectively during the period of maximal developmental plasticity. The results demonstrate that the cat visual cortex at 5 weeks of age is molecularly distinct from the cortex at 15 weeks.