Activation of Notch signaling in human tongue carcinoma
Activation of Notch signaling in human tongue carcinoma
复制标题
人舌癌中Notch信号的激活
DOI：
10.1111/j.1600-0714.2010.00931.x
复制
发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Liao, Gui-Qing
中科院分区：
文献类型：
--
作者：
Zhang, Tong-Han;Liu, Hai-Chao;Liao, Gui-Qing
背景：Notch信号通路在多种肿瘤中的作用是众所周知的，但其在舌鳞状细胞癌中的作用仍不清楚。材料与方法：采用免疫组化和RT-PCR方法检测74例舌癌组织及癌旁组织中Notch 1、Notch 3、Jagged 1和Jagged 2的表达。结果：Notch 1、Notch 3、Jagged 1和Jagged 2在舌癌组织及癌旁组织中均有表达。舌癌组织中mRNA的表达水平明显高于癌旁组织（P < 0.05）。免疫组化结果显示Notch信号分子在Tca 8113、舌癌及癌旁组织中均有表达。Notch 1和Notch 3蛋白在舌癌组织中的表达率均高于癌旁组织（χ 2 = 6.10，P = 0.013; χ 2 = 3.94，P = 0.047）。Notch 1和jagged 1在淋巴结转移阳性的舌癌中表达显著增高（χ 2 = 6.108，P = 0.013; χ 2 = 7.354，P = 0.025）。结论：Notch受体和配体在舌癌及癌旁组织中的表达提示Notch信号通路可能调控舌癌细胞的分化和增殖。Notch信号的异常可能是舌癌发生发展的机制之一。
Background:Involvement of Notch signaling in several tumors is well known, but its role in tongue squamous cell carcinoma remains poorly characterized. The purpose of this study was to evaluate the roles of Notch signaling in the oncogenesis of tongue carcinoma.Materials and Methods:Tumor specimens and adjacent non-neoplastic tongue tissues from 74 patients with tongue carcinoma and human tongue carcinoma cell line Tca8113 were examined using immunohistochemistry and RT-PCR to determine the expressions of Notch1, Notch3, Jagged1, and Jagged2.Results:The mRNA expressions of Notch1, Notch3, Jagged1, and Jagged2 were detected in Tca8113, tongue carcinoma, and adjacent non-neoplastic tongue tissues. The expression levels of mRNAs in tongue carcinoma were higher than those in adjacent non-neoplastic tongue tissues (P < 0.05). Immunohistochemical examination showed that the Notch signal molecules were expressed in Tca8113, tongue carcinoma, and adjacent non-neoplastic tongue tissues. The expression rates of Notch1 and Notch3 protein in tongue carcinoma were higher than those in adjacent non-neoplastic tongue tissues (chi 2 = 6.10, P = 0.013; chi 2 = 3.94, P = 0.047). Notch1 and jagged1 were significantly more highly expressed in lymph node metastasis-positive tongue carcinoma (chi 2 = 6.108, P = 0.013; chi 2 = 7.354, P = 0.025). In addition, expressions of Notch3 and Jagged2 were highly correlated in tongue carcinoma tissues (chi 2 = 42.130, P < 0.001).Conclusions:Expressions of Notch receptors and ligands in tongue carcinoma and adjacent non-neoplastic tongue tissues suggest that Notch signaling may control cell differentiation and proliferation of carcinoma cells. The disorder of Notch signaling may be a mechanism of the tongue carcinoma development.