The pathways for the removal of acrylamide in model systems using glycine based on the identification of reaction products
The pathways for the removal of acrylamide in model systems using glycine based on the identification of reaction products
复制标题
基于反应产物鉴定的甘氨酸模型系统中丙烯酰胺的去除途径
DOI：
10.1016/j.foodchem.2011.03.051
复制
发表时间：
2011-09-15
期刊：
影响因子：
8.8
通讯作者：
Hu, Xiaosong
中科院分区：
文献类型：
--
作者：
Liu, Jie;Chen, Fang;Hu, Xiaosong
在150 ℃的水模型体系中研究了丙烯酰胺与甘氨酸的反应。经HPLC-MS/MS、IT-TOF和NMR鉴定，主要产物为C5 H10 N2 O3、C8 H15 N3 O 4、C7 H12 N2 O 5和C10 H17 N3 O 6。两种关键中间体均被鉴定为乙醛酸和亚氨基二乙酸。甘氨酸去除丙烯酰胺的途径主要是丙烯酰胺与甘氨酸之间的Michael加成反应，无论是否有甘氨酸的初始氧化。反应物和产物含量的变化为上述途径提供了定量证据。用HPLC-MS/MS鉴定了丙烯酰胺与其它14种氨基酸的加成产物。(C)2011爱思唯尔有限公司保留所有权利。
The reaction between acrylamide and glycine was studied in the aqueous model system heated at 150 degrees C. The main reaction products were identified as C5H10N2O3, C8H15N3O4, C7H12N2O5 and C10H17N3O6 using HPLC-MS/MS. IT-TOF and NMR. Both of the critical intermediates were identified as glyoxylic acid and iminodiacetic acid. The pathways for the removal of acrylamide by glycine were proposed as the Michael addition between acrylamide and glycine with or without the initial oxidation of glycine. The changes in the contents of reactants and products provided quantitative evidence for the above pathways. The addition products between acrylamide and other 14 amino acids were identified by HPLC-MS/MS also. (C) 2011 Elsevier Ltd. All rights reserved.