Integrated analysis of copy number alterations and loss of heterozygosity in human pancreatic cancer using a high-resolution, single nucleotide polymorphism array
Integrated analysis of copy number alterations and loss of heterozygosity in human pancreatic cancer using a high-resolution, single nucleotide polymorphism array
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DOI：
10.1159/000155813
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发表时间：
2008-01-01
期刊：
影响因子：
3.5
通讯作者：
Omata, Masao
中科院分区：
文献类型：
--
作者：
Lin, Lian-Jie;Asaoka, Yoshinari;Omata, Masao
目的：探讨胰腺癌的分子遗传事件。方法：我们使用高密度单核苷酸多态性（SNP）阵列分析了25个已建立的胰腺癌细胞系的全基因组拷贝数改变和杂合性缺失（LOH）。我们使用基因组PCR验证了这些数据，并将其应用于临床样本。结果：在至少1株细胞系中检测到26个纯合缺失区，在9p、18q、17p、8p、13q、6q、3p、6p、22q、9q、12q处发现LOH，且频率高（约50%），与前人研究一致。此外，我们在至少2个细胞系中发现了23个扩增区域，其中包括8个未报道的位点。然后，我们在匹配的胰腺癌和正常组织中检测了8个扩增位点的代表性基因。14例显微解剖组织标本中有1例（7.1%）~ 5例（35.7%）存在扩增。结论：利用高分辨率SNP阵列，我们同时研究了全基因组拷贝数改变和LOH。我们确定了几个新的和微小的基因组扩增，其中包含人类胰腺癌的候选癌基因。版权所有2008 S. Karger AG，巴塞尔
Objective: To chart molecular genetic events in pancreatic cancer. Methods: We analyzed genome-wide copy number alterations and loss of heterozygosity (LOH) in 25 established pancreatic cancer cell lines using a high-density single nucleotide polymorphism ( SNP) array. We verified the data using genomic PCR and applied them to clinical samples. Results: Twenty-six homozygous deletion regions were detected in at least 1 cell line and LOH was found at 9p, 18q, 17p, 8p, 13q, 6q, 3p, 6p, 22q, 9q and 12q with high frequency (> 50%), consistent with a previous study. Moreover, we found 23 amplified regions in at least 2 cell lines, including 8 unreported loci. We then examined representative genes at the 8 amplified loci in matched pairs of pancreatic cancer and normal tissues. The amplification was detected in 1 (7.1%) to 5 (35.7%) of 14 microdissected tissue specimens. Conclusion: Using high-resolution SNP arrays, we studied genome-wide copy number alterations and LOH simultaneously. We identified several novel and minute genomic amplifications, which contained candidate oncogenes in human pancreatic cancers. Copyright (C) 2008 S. Karger AG, Basel.