Silencing of an anther-specific zinc-finger gene, MEZ1, causes aberrant meiosis and pollen abortion in petunia
Silencing of an anther-specific zinc-finger gene, MEZ1, causes aberrant meiosis and pollen abortion in petunia
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DOI：
10.1007/s11103-006-0020-0
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发表时间：
2006-06-01
影响因子：
5.1
通讯作者：
Takatsuji, Hiroshi
中科院分区：
文献类型：
--
作者：
Kapoor, Sanjay;Takatsuji, Hiroshi
MEZ1(MEZ1)是首次从矮牵牛中分离到的一个花药特异的基因。在本研究中，我们报道了它的功能特征，包括它在MEZ1沉默植物中的时空表达谱和表型。MEZ1转录本在花药发育早期特异定位于花粉母细胞，随后分布在花药营养组织中。通过共抑制沉默MEZ1导致了雄性减数分裂过程中的几个异常，包括染色体不能浓缩，减数分裂同步性丧失，以及过早和明显不受控制的细胞动力学事件。因此，在减数分裂结束时，MEZ1沉默的小细胞中形成了DNA含量不同的8-10个细胞，而不是正常的4个。大多数细胞过早流产，成熟的细胞具有独特的形态。MEZ1基因沉默的植株在用野生型花粉授粉时是雌性不育的，但它们在自花授粉时很少产生少量种子。由此产生的T-1植株在雄性减数分裂过程中倍性水平增加，并表现出严重的异常，使其完全不育。我们讨论了MEZ1在植物减数分裂过程中的可能作用。
MEZ1 (M Eiosis-associated Zinc-finger protein 1) was first isolated as an anther-specific cDNA from Petunia hybrida. In the present study, we report its functional characterization, including its spatial and temporal expression profiles and phenotypes in MEZ1-silenced plants. MEZ1 transcripts were specifically localized in pollen mother cells during early stages of anther development, and were later distributed in vegetative tissues in anthers. Silencing of MEZ1 by cosuppression resulted in several anomalies during male meiosis that included inability of chromosomes to condense, loss of meiotic synchrony, and premature and apparently uncontrolled cytokinetic events. Consequently, by the end of meiosis 8-10 cells, instead of the normal 4, with varying DNA contents were formed in the MEZ1-silenced meiocytes. Most of these aborted prematurely, and those that matured had a distinctive morphology. MEZ1-silenced plants were female sterile when pollinated with wild-type pollen but they infrequently produced a few seeds upon self-pollination. Resulting T-1 plants had increased ploidy levels and exhibited severe anomalies during male meiosis, rendering them completely sterile. We discuss possible role of MEZ1 in the proper progression of plant meiosis.