Resistance mechanisms of multiresistant serotype 012 Pseudomonas aeruginosa isolated in Europe.
Resistance mechanisms of multiresistant serotype 012 Pseudomonas aeruginosa isolated in Europe.
复制标题
欧洲分离的多重耐药血清型 012 铜绿假单胞菌的耐药机制。
DOI：
10.1093/jac/26.3.319
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
N. Legakis
中科院分区：
文献类型：
--
作者：
T. Pitt;D. Livermore;G. Miller;A. Vatopoulos;N. Legakis
对庆大霉素（MIC大于4 mg/l）和卡比西林（MIC大于128 mg/l）耐药的血清型012铜绿假单胞菌在欧洲广泛存在，并且在表型和遗传特性上是均匀的。有人认为，这种血清型的单一多重耐药菌株已经广泛传播。本研究检测了来自奥地利、比利时、法国、希腊、意大利、荷兰、西德和英国的多重耐药血清型012铜绿假单胞菌的耐药机制。散布的分离株产生PSE-1型β -内酰胺酶，这与它们对这种酶的已知底物（卡比西林、唑洛西林和头孢菌素）的耐药性有关。这些分离物还与氨基糖苷修饰酶AAC(6’)I和ANT(3’)的基因探针反应。已知AAC（6’）I探针与AAC（6’）II基因发生交叉反应。这些微生物对奈替米星、庆大霉素、西索霉素和托布霉素都有耐药性，但对阿米卡星的耐药性较弱，这表明产生的是后者而不是AAC（6’）I。PSE-1 β -内酰胺酶和AAC（6’）I/AAC（6’）II基因在对β -内酰胺类和氨基糖苷敏感的国际抗原分型方案012参考菌株中缺失，1987年在伦敦烧伤单位分离的多耐药血清型012菌株中也缺失。这些生物在表型特性上已被证明与播散性多耐药菌株不同。两株012菌株似乎对阿米卡星和异帕霉素有额外的耐药决定因素。
Serotype 012 Pseudomonas aeruginosa resistant to gentamicin (MIC greater than 4 mg/l) and carbenicillin (MIC greater than 128 mg/l) occur widely in Europe and are homogeneous in their phenotypic and genetic properties. It has been suggested that a single multiresistant strain of this serotype has become widespread. This study examined the resistance mechanisms present in multiresistant serotype 012 P. aeruginosa isolates from Austria, Belgium, France, Greece, Italy, Holland, West Germany and the UK. Disseminated isolates produced a PSE-1 type beta-lactamase, correlating with their resistance to the known substrates of this enzyme (carbenicillin, azlocillin and cefsulodin). These isolates also reacted with gene probes for the aminoglycoside modifying enzymes AAC(6')I and ANT(3'). The probe for AAC(6')I is known to cross-react with the gene for AAC(6')II. The fact that the organisms were resistant to netilmicin, gentamicin, sisomicin and tobramycin, but less so to amikacin, suggested that the latter enzyme was produced rather than AAC(6')I. PSE-1 beta-lactamase and the gene for AAC(6')I/AAC(6')II were absent from the International Antigenic Typing Scheme 012 reference strain, which was sensitive to beta-lactams and aminoglycosides, and also from a multiresistant serotype 012 strain isolated at a London burns unit in 1987. These organisms have been shown previously to be distinct from the disseminated multiresistant strain in their phenotypic properties. Two 012 isolates appeared to have additional resistance determinants to amikacin and isepamicin.