A late-phase, long-term memory trace forms in the γ neurons of Drosophila mushroom bodies after olfactory classical conditioning.
A late-phase, long-term memory trace forms in the γ neurons of Drosophila mushroom bodies after olfactory classical conditioning.
复制标题
DOI：
10.1523/jneurosci.1882-10.2010
复制
发表时间：
2010-12-08
期刊：
影响因子：
--
通讯作者：
Davis RL
中科院分区：
文献类型：
--
作者：
Akalal DB;Yu D;Davis RL
利用体内功能光学成像，我们证明了当气味或电击刺激给果蝇时，果蝇γ蘑菇体(MB)神经元对轴突钙内流做出反应。当果蝇在训练后的几个不同时间进行测试时，在单一训练试验或多个集体训练试验中气味和电击刺激的配对未能改变气味诱发的钙信号。相比之下，在训练后18至48小时之间进行测试时，接受多个但间隔的气味-电击配对的动物显示出流入MB轴突的钙离子显著增加。γ神经元记忆轨迹的这一时间窗口相对于在αMB神经元的α/β分支训练后9至24小时之间发生的修改而移位。α/β和γ神经元的长期记忆痕迹都被转录因子CREB或CaMKII发夹RNA的抑制物阻断。这些结果表明，行为长期嗅觉记忆编码为在训练后重叠但不同的时间窗期间钙离子流入不同的MB神经元的改变。
Using functional optical imaging in vivo, we demonstrate that the γ mushroom body (MB) neurons of Drosophila melanogaster respond with axonal calcium influx when odors or electric shock stimuli are presented to the fly. Pairing of odor and electric shock stimuli in a single training trial or multiple, massed training trials failed to modify the odor-evoked calcium signal when flies were tested at several different times after training. In contrast, animals that received multiple but spaced odor-shock pairings exhibited a robust increase in calcium influx into the MB axons when tested between 18 and 48 hr after training. This time window for the γ neuron memory trace is displaced relative to the modifications that occur between 9 and 24 hr after training in the α branch of the α/β MB neurons. The α/β and the γ neuron long-term memory traces were both blocked by expressing a repressor of the transcription factor Creb or a CaMKII hairpin RNA. These results demonstrate that behavioral long-term olfactory memory is encoded as modifications of calcium influx into distinct MB neurons during overlapping but different windows of time after training.