Myosin-Va mediates RNA distribution in primary fibroblasts from multiple organs.
Myosin-Va mediates RNA distribution in primary fibroblasts from multiple organs.
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DOI：
10.1002/cm.20272
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发表时间：
2008-05
影响因子：
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通讯作者：
Verônica P. Salerno;A. Calliari;D. William Provance;J. Sotelo-Silveira;José Roberto Sotelo;John A. Mercer-John-A
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文献类型：
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作者：
Verônica P. Salerno;A. Calliari;D. William Provance;J. Sotelo-Silveira;José Roberto Sotelo;John A. Mercer-John-A
Myosin-Va has been shown to have multiple functions in a variety of cell types, including a role in RNA transport in neurons.使用来自年轻稀致死(Myo5a(d-l)/Myo5a(d-l))无效突变小鼠和野生型对照的器官的细胞的原代培养物，我们表明在一些但不是全部组织中，纯合无效突变细胞中的RNA分布显着不同。 RNA 定位对肌球蛋白-Va 的依赖性与肌球蛋白-Va 尾部大脑特异性剪接模式的相对丰度相关。我们还表明，肌球蛋白-Va 在合成后不久就参与了 RNA 定位，因为对于超过 30 分钟的 RNA，其缺失的影响会减弱。 Finally, we show that localization of beta-actin mRNA is significantly changed by the absence of myosin-Va. These results suggest that myosin-Va is involved in a transient transport or tethering function in the perinuclear region.
Myosin-Va has been shown to have multiple functions in a variety of cell types, including a role in RNA transport in neurons. Using primary cultures of cells from organs of young dilute-lethal (Myo5a(d-l)/Myo5a(d-l)) null mutant mice and wild-type controls, we show that in some, but not all, tissues, RNA distribution is dramatically different in the homozygous null mutant cells. The dependence of RNA localization on myosin-Va correlates with the relative abundance of the brain-specific splicing pattern of the myosin-Va tail. We also show that myosin-Va is involved in RNA localization soon after synthesis, because the effects of its absence are diminished for RNAs that are more than 30 min old. Finally, we show that localization of beta-actin mRNA is significantly changed by the absence of myosin-Va. These results suggest that myosin-Va is involved in a transient transport or tethering function in the perinuclear region.