Effect of hypercarbia on surface proteins of cultured bovine endothelial cells.
Effect of hypercarbia on surface proteins of cultured bovine endothelial cells.
复制标题
高碳酸对培养牛内皮细胞表面蛋白的影响。
DOI：
10.1152/ajplung.1997.273.6.l1141
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Farber,HW
中科院分区：
文献类型：
--
作者：
Rounds,S;Piggott,D;Dawicki,DD;Farber,HW
高碳酸血症是呼吸衰竭的常见并发症，“允许性高碳酸血症”技术用于治疗机械通气时气道峰压升高的患者，导致动脉P CO2升高 .我们研究了高碳酸对培养的牛主动脉和主肺动脉内皮细胞表面蛋白的影响，使用与乳胶微球结合的乳过氧化物酶评估细胞表面碘化。我们发现，暴露于10%CO2 4小时增加了显示的物质的表观分子量为27，47，和52 kDa。酸性介质不能模拟这种效应。主肺动脉内皮细胞单层的洗涤剂提取物的Western印迹未显示在高碳酸条件下孵育后碳酸酐酶IV（分子量= 52 kDa）的表达增加。高碳酸血症没有改变[35S]蛋氨酸掺入内皮细胞蛋白的模式。我们得出结论，高碳酸血症的4小时持续时间的变化碘化内皮细胞表面蛋白。我们推测，这种影响可能与顶端细胞膜相关蛋白的分泌或显示的变化。
Hypercarbia is a common complication of respiratory failure, and the technique of “permissive hypercapnia” is used to ventilate individuals with increased peak airway pressures on mechanical ventilators, resulting in elevated arterial P CO 2 . We studied the effects of hypercarbia on cultured bovine aortic and main pulmonary artery endothelial cell surface proteins, assessing cell surface iodination using lactoperoxidase bound to latex microspheres. We found that 4 h of exposure to 10% CO2increased the display of substances of apparent molecular masses of 27, 47, and 52 kDa. This effect was not mimicked by acidotic media. Western blots of detergent extracts of main pulmonary artery endothelial cell monolayers did not show increased expression of carbonic anhydrase IV (molecular mass = 52 kDa) after incubation under hypercarbic conditions. Hypercarbia did not change the pattern of [35S]methionine incorporation into endothelial cell proteins. We conclude that hypercarbia of 4-h duration changes iodinated endothelial cell surface proteins. We speculate that this effect may be related to changes in secretion or display of apical cell membrane-associated proteins.