Nonspecific T-cell homing during inflammation in atopic dermatitis: Expression of cutaneous lymphocyte-associated antigen and integrin alpha E beta 7 on skin-infiltrating T cells
Nonspecific T-cell homing during inflammation in atopic dermatitis: Expression of cutaneous lymphocyte-associated antigen and integrin alpha E beta 7 on skin-infiltrating T cells
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DOI：
10.1016/s0091-6749(97)70175-1
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发表时间：
1997-11-01
影响因子：
14.2
通讯作者：
Thepen, T
中科院分区：
文献类型：
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作者：
deVries, IJM;LangeveldWildschut, EG;Thepen, T
特应性皮炎 (AD) 是一种慢性皮肤病，其特征是活化的记忆 CD4(+) T 细胞浸润到皮肤中。研究 AD 患者过敏性炎症发作的模型是特应性斑贴试验 (APT)，其中通过表皮应用空气过敏原，50% 的过敏性 AD 患者会诱发湿疹反应。 T 细胞外渗到皮肤中被认为关键取决于表面分子皮肤淋巴细胞相关抗原 (CLA) 的表达，CLA 识别并结合内皮上的配体 E-选择素。我们通过对皮肤活检标本进行免疫组织化学双染色，研究了 AD 患者皮肤 T 细胞上的 CLA 和肠道归巢受体 α E β 7 (HML-1) 以及 APT 反应和十二烷基硫酸镍钠斑贴试验反应中的表达动态。结果显示，与相同患者和非特应性个体的非病变皮肤相比，患有AD、APT反应以及十二烷基硫酸镍和钠斑贴试验反应的患者病变皮肤中CD3(+) T细胞的数量增加。相比之下，AD患者皮损、APT反应、十二烷基硫酸钠和镍斑贴试验反应中CLA(+) T细胞的百分比下降。此外，我们发现皮肤中 T 细胞显着表达 alpha E beta 7，表明过敏性皮肤炎症期间 T 细胞非特异性流入。我们认为，在过敏性皮肤炎症期间，CLA 表达并不是皮肤 T 细胞浸润的先决条件。 CLA 表达对于免疫监视过程中 T 细胞从血液渗入皮肤或过敏原特异性 T 细胞保留在皮肤中可能很重要。
Atopic dermatitis (AD) is a chronic skin disorder, characterized by infiltration of activated memory CD4(+) T cells into skin. A model to study the onset of allergic inflammation in a patient with AD is the atopy patch test (APT), in which, by epicutaneous application of aeroallergen, an eczematous reaction is induced in 50% of sensitized patients with AD. Extravasation of T cells into skin is thought to be critically dependent on expression of the surface molecule cutaneous lymphocyte-associated antigen (CLA), which recognizes and binds its ligand E-selectin on endothelium. We studied the dynamics of expression of CLA and the gut homing receptor alpha E beta 7 (HML-1) on T cells in the skin of patients with AD and in APT reactions and nickel and sodium lauryl sulfate patch test reactions by means of immunohistochemical double staining of skin biopsy specimens. The results show an increase in the number of CD3(+) T cells in the lesional skin of patients with AD, APT reactions, and nickel and sodium lauryl sulfate patch test reactions as compared with nonlesional skin of the same patients and nonatopic individuals. In contrast, the percentages of CLA(+) T cells in the lesional skin of patients with AD, in the APT reactions, and in sodium lauryl sulfate and nickel patch test reactions were decreased. In addition, we found a marked expression of alpha E beta 7 by T cells present in skin, indicating a nonspecific influx of T cells during allergic skin inflammation. We propose that during allergic skin inflammation CLA expression is not a prerequisite for cutaneous T-cell infiltration. CLA expression may be important for T cells to extravasate from blood into skin during immune surveillance or for retention of allergen-specific T cells in skin.