hTERT can function with rabbit telomerase RNA: regulation of gene expression and attenuation of apoptosis.
hTERT can function with rabbit telomerase RNA: regulation of gene expression and attenuation of apoptosis.
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hTERT 可以与兔端粒酶 RNA 一起发挥作用：调节基因表达并减弱细胞凋亡。
DOI：
10.1006/bbrc.2000.3834
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发表时间：
2000
影响因子：
3.1
通讯作者：
Li,DW
中科院分区：
文献类型：
--
作者：
Xiang,H;Wang,J;Mao,YW;Li,DW
端粒酶是一种特殊的 DNA 聚合酶，可将端粒序列添加到染色体末端。功能性端粒酶复合物包含端粒酶逆转录酶（TERT）和端粒酶RNA（TR）。尽管众所周知，人端粒酶逆转录酶 (hTERT) 可以在不同的人类细胞系中良好发挥作用，但尚未证明它是否与其他物种的端粒酶模板 RNA 兼容。在这里，我们报告表达的 hTERT 与兔端粒酶模板 RNA (rTR) 功能相容，如 TRAP 测定所证明的。 hTERT 和 rTR 之间的直接相互作用通过免疫沉淀连接的 RT-PCR 得到进一步证实，其中 rTR 从抗 hTERT 抗体免疫沉淀的复合物中检测到。兔晶状体上皮细胞中表达的hTERT表现出两个主要功能：调节其他基因的表达和减弱细胞凋亡。因此，端粒酶除了端粒合成之外还具有多种功能，并且模板RNA在人和兔之间在功能上是保守的。
Telomerase is a specialized DNA polymerase that adds telomeric sequences onto chromosome ends. The functional telomerase complex contains a telomerase reverse transcriptase (TERT) and also a telomerase RNA (TR). Although it is well established that the human telomerase reverse transcriptase (hTERT) can function well in different human cell lines, it has not been shown whether it is compatible with telomerase template RNA from other species. Here we report that the expressed hTERT is functionally compatible with rabbit telomerase template RNA (rTR) as demonstrated by TRAP assay. The direct interaction between hTERT and rTR is further confirmed by immunoprecipitation-linked RT-PCR in which rTR is detected from the complex immunoprecipitated by an anti-hTERT antibody. The hTERT expressed in rabbit lens epithelial cells demonstrates two major functions: modulation of expression of other genes and attenuation of apoptosis. Thus, telomerase has a variety of functions besides telomere synthesis, and the template RNA is functionally conserved between human and rabbit.