LYSIS OF DENGUE VIRUS-INFECTED CELLS BY NATURAL CELL-MEDIATED CYTO-TOXICITY AND ANTIBODY-DEPENDENT CELL-MEDIATED CYTO-TOXICITY
LYSIS OF DENGUE VIRUS-INFECTED CELLS BY NATURAL CELL-MEDIATED CYTO-TOXICITY AND ANTIBODY-DEPENDENT CELL-MEDIATED CYTO-TOXICITY
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DOI：
10.1128/jvi.52.1.223-230.1984
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
ENNIS, FA
中科院分区：
文献类型：
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作者：
KURANE, I;HEBBLEWAITE, D;ENNIS, FA
无登革2病毒抗体的人外周血单个核细胞（PBMC）对登革2病毒感染的Raji细胞的裂解程度明显高于未感染的Raji细胞。小鼠抗登革热抗体的加入增加了PBMC对登革热感染的Raji细胞的裂解。登革热2型免疫的人血清也增加了PBMC对登革热感染的Raji细胞的裂解。这些结果表明，pmc介导的细胞毒性和抗体依赖细胞介导的细胞毒性（ADCC）都能引起登革热感染的Raji细胞的显著裂解。在ADCC实验中，感染Raji细胞的裂解与加入登革热特异性抗体的稀释度相关，表明登革热病毒感染Raji细胞的裂解具有登革热病毒特异性。在PBMC和登革热感染的Raji细胞培养上清液中检测到α干扰素（ifn . α）。PBMC对登革热感染的Raji细胞的裂解增强可能不是由于IFN的产生，因为所有IFN的活性都被抗IFN. α中和。抗体不降低登革热感染细胞和外源性ifn . α预处理的效应细胞的裂解。与未感染的细胞相比，感染登革热的细胞裂解程度更高。在自然杀伤和ADCC实验中分析了负责裂解登革热病毒感染的Raji细胞的效应细胞。未贴壁的PBMC比贴壁细胞引起更多的溶解。用单克隆抗体对非贴壁细胞进行鉴定表明，在自然杀伤和ADCC检测中，主要的效应细胞包含在OKM1+和OKT3-部分。
Peripheral blood mononuclear cells (PBMC) from humans without antibodies to dengue 2 virus lysed dengue 2 virus-infected Raji cells to a significantly greater degree than uninfected Raji cells. The addition of mouse anti-dengue antibody increased the lysis of dengue-infected Raji cells by PBMC. Dengue 2 immune human sera also increased lysis of dengue-infected Raji cells by PBMC. These results indicate that both PBMC-mediated cytotoxicity and antibody-dependent cell-mediated cytotoxicity (ADCC) can cause significant lysis of dengue-infected Raji cells. The lysis of infected Raji cells in the ADCC assay correlated with the dilution of dengue-specific antibody which was added, indicating the dengue virus specificity of the lysis of dengue virus-infected Raji cells. Alpha interferon (IFN.alpha.) was detected in the culture supernatant of PBMC and dengue-infected Raji cells. Enhanced lysis of dengue-infected Raji cells by PBMC may not be due to the IFN produced, because neutralization of all IFN activity with anti-IFN.alpha. antibody did not decrease the lysis of dengue-infected cells, and effector cells pretreated with exogenous IFN.alpha. also lysed dengue-infected cells to a greater degree than uninfected cells. The effector cells responsible for lysis of dengue virus-infected Raji cells in the natural killer and ADCC assays were analyzed. Nonadherent PBMC caused more lysis than did adherent cells. Characterization of nonadherent cells with monoclonal antibodies showed that the predominant responsible effector cells were contained in OKM1+ and OKT3- fraction in the natural killer and ADCC assays.