C2H2 transcription factor brlA regulating conidiation and affecting growth and biosynthesis of secondary metabolites in Aspergillus clavatus
C2H2 transcription factor brlA regulating conidiation and affecting growth and biosynthesis of secondary metabolites in Aspergillus clavatus
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C2H2转录因子brlA调节棒曲霉分生孢子并影响生长和次生代谢产物生物合成
DOI：
10.17957/ijab/15.0813
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发表时间：
2018
影响因子：
--
通讯作者：
Donghua Jiang
中科院分区：
文献类型：
--
作者：
Xiaofei Han;Chuxuan Xu;Qi Zhang;Bei Jiang;Jieshi Zheng;Donghua Jiang
Aspergillus clavatus is a promising candidate suited for industrial production with its capacity of producing a variety of important metabolites. However, the research of gene functions in A. clavatus is still stagnant. Cloning a 1290 bp brlA gene, sequence analysis revealed that it encoded a C2H2 type transcription factor in A. clavatus. Deletion of the gene by building a brlA-deleted plasmid, numerous significant alterations occurred in growth, colonial morphology, microstructure, and conidiation. The HPLC analysis showed that secondary metabolism was also changed in the brlA-deleted mutant. The results indicated that brlA gene regulated conidiation and it was also involved in the regulation of growth and biosynthesis of secondary metabolites in A. clavatus.
