Nucleotide sequence, genome organization and phylogenetic analysis of Strawberry pallidosis associated virus, a new member of the genus Crinivirus
Nucleotide sequence, genome organization and phylogenetic analysis of Strawberry pallidosis associated virus, a new member of the genus Crinivirus
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DOI：
10.1007/s00705-004-0410-z
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发表时间：
2005-02
影响因子：
2.7
通讯作者：
I. Tzanetakis;J. Reed;R. Martin
中科院分区：
文献类型：
--
作者：
I. Tzanetakis;J. Reed;R. Martin
草莓苍白病相关病毒（Strawberry pallidosis associated virus，SPaV）是一种新发现的梭状病毒科（Closteroviridae）Crinivirus属病毒。RNA 1长8067个核苷酸，编码至少3个开放阅读框（ORF）。第一个ORF（ORF 1a）指定了一个多功能蛋白，具有木瓜蛋白酶样蛋白酶，甲基转移酶和RNA解旋酶结构域。RNA依赖性RNA聚合酶在ORF 1b中编码，可能通过+1核糖体移码表达。RNA 1的3′ ORF编码一个具有两个潜在跨膜螺旋的小蛋白。RNA 2长7979个核苷酸，编码8个ORF，其氨基酸序列和排列与其他海百合病毒相似。SPaV编码迄今为止测序的梭状病毒的最大结构蛋白，因为病毒的次要外壳蛋白具有约80 kDa的分子量。3′非翻译区的核苷酸序列同源性约为56%，预测的非翻译区的折叠相似。系统发育分析表明，SPaV与Abutilon yellows virus和Beet pseudo-yellows virus的亲缘关系最近，后者是最近发现的在草莓指示植物上引起与SPaV相同症状的病毒。
The complete nucleotide sequence of Strawberry pallidosis associated virus (SPaV), a newly identified member of the genusCrinivirus, familyClosteroviridaehas been determined. RNA 1 is 8067 nucleotides long and encodes at least three open reading frames (ORFs). The first ORF (ORF 1a) specifies a multifunctional protein that has papain-like proteinase, methyltransferase and RNA helicase domains. The RNA-dependent-RNA polymerase is encoded in ORF 1b and is probably expressed by a +1 ribosomal frameshift. The 3′ ORF of RNA 1 encodes a small protein with two potential transmembrane helices. RNA 2 is 7979 nucleotides long and encodes 8 ORFs, similar in amino acid sequence and arrangement with those of other criniviruses. SPaV encodes the largest structural protein of closteroviruses sequenced to date as the minor coat protein of the virus has molecular mass of approximately 80 kDa. The 3′ non-translated regions share nucleotide sequence identities of about 56% and the predicted folding of the non-translated regions is similar. Phylogenetic analyses reveal that SPaV is related most closely toAbutilon yellows virusandBeet pseudo-yellows virus, another virus that has been identified recently to cause identical symptoms on strawberry indicator plants as SPaV.