Insight Into Host-Microbe Interactions Using Microbial Flow Cytometry Coupled to Next-Generation Sequencing.
Insight Into Host-Microbe Interactions Using Microbial Flow Cytometry Coupled to Next-Generation Sequencing.
复制标题
使用微生物流式细胞术与下一代测序相结合深入了解宿主-微生物相互作用。
DOI：
10.1093/jpids/piab092
复制
发表时间：
2021
影响因子：
3.2
通讯作者：
Green,JamalL
中科院分区：
文献类型：
--
作者：
Silverman,MichaelA;Green,JamalL
100多年来，基于抗体的检测一直是传染病诊断的基石。这些检测依赖于对几乎所有感染的体液反应的敏感性和特异性。虽然下一代测序（NGS）通过直接识别感染性微生物的核酸来改善诊断和揭示宿主-微生物关系具有巨大的潜力，但新方法仍然存在挑战和机遇。在这里，我们回顾了一组将抗体反应与抗体标记微生物和NGS的流式细胞术相结合的前沿技术。这些研究使我们的免疫系统与内源性微生物群落之间的动态关系成为焦点，而内源性微生物群落是病原体的重要来源。为了简单起见，我们使用总称mFLOW-Seq（微生物流式细胞术与NGS耦合）来描述这些方法。
Antibody-based assays have been a cornerstone of infectious disease diagnostics for over 100 years . These assays rely on the exquisite sensitivity and specificity of humoral response to almost all infections. While next-generation sequencing (NGS) has tremendous potential to improve diagnostics and uncover host-microbial relationships by directly identifying nucleic acids from infectious microbes, challenges and opportunities for new approaches remain. Here, we review a group of cutting-edge techniques that couple antibody responses with flow cytometry of antibody tagged microbes and NGS. These studies are bringing into focus the dynamic relationship between our immune systems and endogenous microbial communities, which are an important source of pathogens. For simplicity, we use the umbrella term mFLOW-Seq (microbial flow cytometry coupled to NGS) to describe these approaches.