Penetration of living cell membranes with fortified carbon nanotube tips.
Penetration of living cell membranes with fortified carbon nanotube tips.
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DOI：
10.1021/la701878n
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发表时间：
2007-09
期刊：
影响因子：
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通讯作者：
I. Vakarelski;Scott C. Brown;K. Higashitani;B. Moudgil
中科院分区：
文献类型：
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作者：
I. Vakarelski;Scott C. Brown;K. Higashitani;B. Moudgil
我们通过修改多壁碳纳米管（MCNT）端部原子力显微镜（AFM）尖端，制造出适合单细胞手术的坚固的纳米手术针。超长的MCNT AFM尖端被制备和强化了碳分子层，以克服机械不稳定性，然后涂上一层金外壳，以提高化学的通用性。最终制备的末端直径约为30-40 nm， MCNT探针的长度为几微米。我们展示了这些修饰的MCNT尖端携带纳米粒子有效载荷的能力，并以目前报道的最小压痕深度（100-200纳米）和最低穿透力（100-200 pN）穿透活胸膜间皮细胞的质膜。
We have fabricated robust nanosurgical needles suitable for single cell operations by modifying multiwalled carbon nanotube (MCNT)-terminated atomic force microscopy (AFM) tips. Extra-long MCNT AFM tips were prepared and fortified with molecular layers of carbon to overcome mechanical instabilities and then coated with an outer shell of gold to promote chemical versatility. The terminal diameters of the final fabricated tips were approximately 30-40 nm, and the MCNT probes were several micrometers in length. We illustrate the capability of these modified MCNT tips to carry nanoparticulate payloads and to penetrate the plasma membrane of living pleural mesothelial cells at the smallest indentation depths (100-200 nm) and lowest penetration forces (100-200 pN) currently reported for these procedures.