Cloning, characterization, and antigen specificity of T-lymphocyte subsets extracted from gingival tissue of chronic adult periodontitis patients
Cloning, characterization, and antigen specificity of T-lymphocyte subsets extracted from gingival tissue of chronic adult periodontitis patients
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从慢性成人牙周炎患者牙龈组织中提取的 T 淋巴细胞亚群的克隆、表征和抗原特异性
作者：
A. Wassenaar;C. Reinhardus;T. Thepen;L. Abraham;F. Kievits
慢性牙周炎的特征是B和T淋巴细胞在牙龈结缔组织内的致密浸润。据报道，不同的厌氧革兰氏阴性菌以及对胶原蛋白的自身免疫在这种疾病的病因和发病机制中起作用。在这里，我们描述了克隆和表征的CD 4+和CD 8 + T淋巴细胞从炎症牙龈组织从4例慢性牙周炎患者。用植物血凝素和白细胞介素-2培养克隆，并测试牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、人I型胶原和两种细菌热休克蛋白的全细胞抗原的增殖。从所有四名患者中获得与I型胶原蛋白反应的CD 4 + T细胞克隆。这些克隆中的80%具有类似于小鼠2型T辅助（Th）表型的表型，即，它们产生高水平的白细胞介素-4和低水平的γ干扰素。未获得I型胶原反应性CD 8+克隆。细菌抗原反应性的CD 4+和/或CD 8 + T细胞克隆也从每个病人，大多数克隆显示出Th 0样细胞因子的模式，并产生等量的白细胞介素-4和γ干扰素。尽管大多数克隆与中间型巴斯德菌反应，但似乎免疫应答并不严格针对这种特定微生物，因为与其他细菌之一反应的克隆也从两名患者中获得。我们提出，胶原特异性CD 4 + Th 2样T细胞有助于慢性牙周炎，但其激活模式可能是由牙周炎相关细菌特异性Th 0样T细胞控制。
Chronic periodontitis is characterized by dense infiltrations of B and T lymphocytes within the gingival connective tissue. Distinct anaerobic gram-negative bacteria as well as autoimmunity to collagen have been reported to play a role in the etiology and the pathogenesis of this disease. Here we describe the cloning and characterization of CD4+ and CD8+ T lymphocytes isolated from inflamed gingival tissue obtained from four patients with chronic periodontitis. Clones were raised with phytohemagglutinin and interleukin-2 and tested for proliferation in response to whole-cell antigens of Porphyromonas gingivalis, Prevotella intermedia, Actinobacillus actinomycetemcomitans, human collagen type I, and two bacterial heat shock proteins. CD4+ T-cell clones reactive with collagen type I were obtained from all four patients. Eighty percent of these clones had phenotypes resembling the mouse type 2 T helper (Th) phenotype, i.e., they produced high levels of interleukin-4 and low levels of gamma interferon. No collagen-type-I-reactive CD8+ clones were obtained. Bacterial-antigen-reactive CD4+ and/or CD8+ T-cell clones were also obtained from each patient, and the majority of the clones showed a Th0-like cytokine pattern and produced equal amounts of interleukin-4 and gamma interferon. Although most clones were reactive with P. intermedia, it seems that the immune response is not strictly directed against this particular microorganism, as clones reactive with one of the other bacteria were also obtained from two patients. We propose that collagen-specific CD4+ Th2-like T cells contribute to the chronicity of periodontitis but that their modes of activation might be controlled by Th0-like T cells specific for periodontitis-associated bacteria.
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DOI：
10.1073/pnas.88.10.4538
发表时间：
1991-05-01
影响因子：
11.1
作者：
PARRONCHI, P;MACCHIA, D;ROMAGNANI, S
通讯作者：
ROMAGNANI, S
影响因子：
56.9
作者：
SALGAME, P;ABRAMS, JS;BLOOM, BR
通讯作者：
BLOOM, BR
影响因子：
3.5
作者：
LISTGARTEN, MA
通讯作者：
LISTGARTEN, MA
成人牙周炎中局部产生的 I 型胶原蛋白自身抗体的免疫球蛋白同种型分布。
影响因子：
6.7
作者：
Jonsson,R;Pitts,A;Lue,C;Gay,S;Mestecky,J
通讯作者：
Mestecky,J
成人牙周病中胶原蛋白的自身免疫。
DOI：
10.1111/j.1600-0714.1988.tb01315.x
发表时间：
1988
期刊：
Journal of oral pathology
影响因子：
--
作者：
Hirsch,HZ;Tarkowski,A;Miller,EJ;Gay,S;Koopman,WJ;Mestecky,J
通讯作者：
Mestecky,J