Isolated liver gap junctions: gating of transjunctional currents is similar to that in intact pairs of rat hepatocytes.
Isolated liver gap junctions: gating of transjunctional currents is similar to that in intact pairs of rat hepatocytes.
复制标题
孤立的肝脏间隙连接：跨连接电流的门控与完整的大鼠肝细胞对相似。
DOI：
10.1073/pnas.83.15.5494
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Hertzberg,EL
中科院分区：
文献类型：
--
作者：
Spray,DC;Saez,JC;Brosius,D;Bennett,MV;Hertzberg,EL
我们以前已经表明，大鼠肝脏间隙连接的电导被阻断的亲和纯化的多克隆抗体产生的大鼠肝脏连接膜，是不受中度transjunctional或跨膜电位，并可逆地降低细胞质酸化和灌注辛醇。我们现在已经记录了电流从孤立的肝脏缝隙连接使用贴片电极浸渍通过一层混合脂质的浓度匹配的孤立的肝脏附着膜。这些电流被相同的多克隆抗体阻断，对移液器吸头上施加的中等电压不敏感，并且被类似浓度的H离子和辛醇可逆地阻断，就像原位连接一样。电流很可能起源于间隙连接;它们被低pH和其他试剂阻断表明门控机制是差距连接本身固有的，推测是通道形成蛋白的构象变化所致。
We have shown previously that conductance of rat liver gap junctions is blocked by an affinity-purified polyclonal antibody generated against rat liver junctional membranes, is not affected by moderate transjunctional or transmembrane potentials, and is reversibly decreased by cytoplasmic acidification and perfusion with octanol. We have now recorded currents from isolated liver gap junctions using patch electrodes dipped through a layer of mixed lipids whose concentrations match those of isolated liver appositional membranes. These currents are blocked by the same polyclonal antibody, are insensitive to moderate voltages imposed across the pipette tip, and are reversibly blocked by similar concentrations of H ions and octanol as are junctions in situ. The currents are likely to be gap junctional in origin; their block by low pH and other agents indicates that the gating mechanisms are intrinsic to the gap junctions themselves and presumably result from conformational change in the channel-forming protein.