Denitrification of groundwater using a sulfur-oxidizing autotrophic denitrifying anaerobic fluidized-bed MBR: performance and bacterial community structure
Denitrification of groundwater using a sulfur-oxidizing autotrophic denitrifying anaerobic fluidized-bed MBR: performance and bacterial community structure
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DOI：
10.1007/s00253-014-6113-9
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发表时间：
2015-03
影响因子：
5
通讯作者：
Lili Zhang;Chao Zhang;Chengzhi Hu;Huijuan Liu;J. Qu
中科院分区：
文献类型：
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作者：
Lili Zhang;Chao Zhang;Chengzhi Hu;Huijuan Liu;J. Qu
本文研究了一种新型硫氧化自养反硝化厌氧床膜生物反应器(AnFB-MBR)，该反应器具有克服传统硫氧化自养反硝化系统局限性的潜力。在水力停留时间为0.5h~5.0h的条件下，以NO3-−-N为25-80 mg/L的人工合成地下水为进水，生物反应器对硝酸盐氮的去除能力达到4.0g/L反应器，出水水质稳定，出水水质稳定。膜生物反应器的膜通量维持在1.5~15g/m−2 h−1范围内，膜清洗周期为35~81 d，对膜生物反应器的运行性能没有明显影响。15N示踪分析表明，氮素转化为15N-N和15N-生物量分别占总氮量的88.1-93.1%和6.4-11.6%。在硫氧化自养反硝化过程中，只有0.3-0.5%的去除氮以15N2O-N的形式存在，从而降低了大量N2O排放的潜在风险。硫氧化自养反硝化细菌联合体主要由变形杆菌、氯杆菌和柔绿藻属的细菌组成，其中硫杆菌属、硫单胞菌和革兰氏菌占主导地位。焦化测序分析还表明，稳定的微生物群落与AnFB-MBR性能的提高相对应。总体而言，本研究描述了相对较高的硝酸盐去除能力，可接受的通量，表明了该技术未来的实践潜力。
This paper investigates a novel sulfur-oxidizing autotrophic denitrifying anaerobic fluidized bed membrane bioreactor (AnFB-MBR) that has the potential to overcome the limitations of conventional sulfur-oxidizing autotrophic denitrification systems. The AnFB-MBR produced consistent high-quality product water when fed by a synthetic groundwater with NO3−–N ranging 25–80 mg/L and operated at hydraulic retention times of 0.5–5.0 h. A nitrate removal rate of up to 4.0 g NO3−-N/Lreactord was attained by the bioreactor, which exceeded any reported removal capacity. The flux of AnFB-MBR was maintained in the range of 1.5–15 L m−2h−1. Successful membrane cleaning was practiced with cleaning cycles of 35–81 days, which had no obvious effect on the AnFB-MBR performance. The15N-tracer analyses elucidated that nitrogen was converted into15N2-N and15N-biomass accounting for 88.1–93.1 % and 6.4–11.6 % of the total nitrogen produced, respectively. Only 0.3–0.5 % of removed nitrogen was in form of15N2O-N in sulfur-oxidizing autotrophic denitrification process, reducing potential risks of a significant amount of N2O emissions. The sulfur-oxidizing autotrophic denitrifying bacterial consortium was composed mainly of bacteria fromProteobacteria,Chlorobi, andChloroflexiphyla, with generaThiobacillus,Sulfurimonas, andIgnavibacterialesdominating the consortium. The pyrosequencing assays also suggested that the stable microbial communities corresponded to the elevated performance of the AnFB-MBR. Overall, this research described relatively high nitrate removal, acceptable flux, indicating future potential for the technology in practice.