FN13762 Murine Breast Cancer: Region-by-Region Correlation of First-Pass Perfusion CT Indexes with Histologic Vascular Parameters
FN13762 Murine Breast Cancer: Region-by-Region Correlation of First-Pass Perfusion CT Indexes with Histologic Vascular Parameters
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DOI：
10.1148/radiol.2513081215
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发表时间：
2009-06-01
期刊：
影响因子：
19.7
通讯作者：
Im, Jung-Gi
中科院分区：
文献类型：
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作者：
Park, Chang Min;Goo, Jin Mo;Im, Jung-Gi
目的：采用逐区域相关方法探讨大鼠FN13762乳腺癌首过灌注CT指标与组织学血管参数之间的相关性。材料和方法：动物护理和使用委员会批准了本研究。将FN13762小鼠乳腺癌细胞植入14只雌性Fischer 344大鼠体内，并进行首过灌注CT。使用商业灌注软件生成描绘血流、血容量、平均通过时间 (MTT) 和渗透性表面积 (PSA) 乘积的 CT 灌注图。将整个肿瘤区域在灌注图上分为6个独立区域，并对区域灌注指数进行量化。在与灌注图相对应的组织学区域中测量组织学血管参数，包括微血管密度（MVD）、管腔血管数量、管腔血管面积和管腔血管周长。对肿瘤区域灌注指标与相应肿瘤区域的组织学血管参数进行相关性分析。此外，整个肿瘤的平均灌注值与肿瘤内热点的组织学血管参数相关。在 14 只大鼠中，4 只被排除在分析之外，结果基于最终总共 10 只大鼠。 结果：与正常颈部肌肉中的这些值相比，肿瘤中的血流量、血容量和 PSA 产物显着更高，MTT 显着更短（全部 P < .05）。在逐个区域相关性中，区域血流量 (r = 0.476)、血容量 (r = 0.348) 和 MTT (r = -0.506) 与相应肿瘤区域的 MVD 显着相关（全部 P < .01）。调整大鼠之间的生物学变异性后，区域血流量（r = 0.614）、血容量（r = 0.515）、MTT（r = -0.524）和PSA产物（r = 0.228）仍然与相应肿瘤区域的MVD显着相关。整个肿瘤的CT灌注指标与热点的组织学血管参数之间的相关性分析未显示出显着的相关性（P = .05）。结论：区域血流、血容量和MTT与相应肿瘤区域的MVD显着相关。 (C) 北美放射学会，2009
Purpose: To investigate the correlation between first-pass perfusion computed tomographic (CT) indexes and histologic vascular parameters in FN13762 breast cancer in rats by using region-by-region correlation methods.Materials and Methods: The Animal Care and Use Committee approved this study. FN13762 murine breast cancer cells were implanted in 14 female Fischer 344 rats, and first-pass perfusion CT was performed. CT perfusion maps depicting blood flow, blood volume, mean transit time (MTT), and permeability-surface area (PSA) product were generated by using commercial perfusion software. The entire tumor area was divided into six separate regions on perfusion maps, and the regional perfusion indexes were quantified. Histologic vascular parameters, including microvessel density (MVD), luminal vessel number, luminal vessel area, and luminal vessel perimeter, were measured in the histologic region corresponding to the perfusion maps. Correlation analysis was performed between regional tumor perfusion indexes and histologic vascular parameters of the corresponding tumor region. Additionally, mean perfusion values of the entire tumor were correlated with histologic vascular parameters of the hot spot within the tumor. Among 14 rats, four were excluded from the analysis, and results were based on a final total of 10 rats.Results: In tumors, blood flow, blood volume, and PSA product were significantly higher and MTT was significantly shorter (P < .05 for all) than these values in normal neck muscles. At region-by-region correlation, regional blood flow (r = 0.476), blood volume (r = 0.348), and MTT (r = -0.506) were significantly correlated with MVD in the corresponding tumor region (P < .01 for all). After adjustment for biologic variability between rats, regional blood flow (r = 0.614), blood volume (r = 0.515), MTT (r = -0.524), and PSA product (r = 0.228) remained significantly correlated with MVD in the corresponding tumor region. Correlation analysis between CT perfusion indexes of the entire tumor and histologic vascular parameters of the hot spot did not show significant correlations (P = .05).Conclusion: Regional blood flow, blood volume, and MTT are significantly correlated with MVD in the corresponding tumor region. (C) RSNA, 2009