Coexpression of Fc gamma receptor IIIA and interleukin-2 receptor beta chain by a subset of human CD3+/CD8+/CD11b+ lymphocytes.
Coexpression of Fc gamma receptor IIIA and interleukin-2 receptor beta chain by a subset of human CD3+/CD8+/CD11b+ lymphocytes.
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Fc γ 受体 IIIA 和白细胞介素 2 受体 β 链由人 CD3 /CD8 /CD11b 淋巴细胞亚群共表达。
DOI：
10.1007/bf00919976
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发表时间：
1993
影响因子：
9.1
通讯作者：
Ferrarini,M
中科院分区：
文献类型：
--
作者：
Zupo,S;Azzoni,L;Massara,R;D'Amato,A;Perussia,B;Ferrarini,M
在这项研究中，我们鉴定和表征了人类外周血T细胞的一个子集，存在于所有个体中，具有先前单独或在特殊情况下描述的T细胞的特征。这些细胞存在于静息外周血淋巴细胞内CD8+T淋巴细胞的纯化悬浮液中，表达αβ T细胞受体（TCR），由于表面CD11b （TCRαβ+/CD3+/CD8+/CD11b+细胞）的高密度表达，可以被鉴定和分离。它们组成性地共表达IL-2受体β链、FcγRIIIA和CD56。虽然CD3+/CD8+/CD11b+细胞不介导自发细胞毒性，但它们具有细胞毒性潜能，在抗fc γ riii （CD16）或抗CD3单克隆抗体存在的P815靶细胞的重定向细胞毒性试验中得到证实。用IL-2刺激CD3+/CD8+/CD11b+细胞可诱导增殖，对nk敏感和nk耐药靶细胞产生细胞毒性，并表达表面活化抗原，包括IL-2受体α链（CD25）。通过在il -2和自体饲养细胞存在下对纯化的CD3+/CD8+/CD16+/CD56+细胞进行限稀释克隆，获得了具有细胞毒功能的CD3+/CD8+/CD16+/CD56+细胞克隆，包括表面CD16介导的细胞毒功能。我们的数据支持这样的假设，即CD3+/CD8+/CD11b+/CD16+细胞代表一个离散的外周血淋巴细胞亚群，可能是在几种病理条件下和大颗粒淋巴细胞增多症中扩张的生理对应体。
In this study we identify and characterize a subset of human peripheral blood T cells, present in all individuals, that has features previously described for T cells either separately or in special circumstances. These cells are found in purified suspensions of resting peripheral blood lymphocytes within the CD8+T lymphocytes, express αβ T cell receptor (TCR), and can be identified and isolated because of high-density expression of surface CD11b (TCRαβ+/CD3+/CD8+/CD11b+cells). They coexpress constitutively the IL-2 receptor β chain, FcγRIIIA, and CD56. Although they do not mediate spontaneous cytotoxicity, CD3+/CD8+/CD11b+cells have cytotoxic potential, demonstrated in redirected cytotoxicity assays with P815 target cells in the presence of anti-FcγRIII (CD16) or anti-CD3 monoclonal antibodies. Stimulation of CD3+/CD8+/CD11b+cells with rIL-2 induces proliferation, cytotoxicity against NK-sensitive and NK-resistant target cells, and expression of surface activation antigens, including IL-2 receptor α chain (CD25). CD3+/CD8+/CD16+/CD56+cell clones with cytotoxic functions including those mediated by engagement of surface CD16 were obtained by limiting-dilution cloning of purified CD3+/CD8+/CD11b+cells in the presence of rIL-2 and autologous feeder cells. Our data support the hypothesis that the CD3+/CD8+/CD11b+/CD16+cells represent a discrete peripheral blood lymphocyte subset that could be the physiological counterpart of that expanded in several pathological conditions and in large granular lymphocyte lymphocytosis.