A FUNCTIONAL T3 MOLECULE ASSOCIATED WITH A NOVEL HETERODIMER ON THE SURFACE OF IMMATURE HUMAN THYMOCYTES
A FUNCTIONAL T3 MOLECULE ASSOCIATED WITH A NOVEL HETERODIMER ON THE SURFACE OF IMMATURE HUMAN THYMOCYTES
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DOI：
10.1038/322179a0
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发表时间：
1986-07-10
期刊：
影响因子：
64.8
通讯作者：
CHESS, L
中科院分区：
文献类型：
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作者：
BANK, I;DEPINHO, RA;CHESS, L
参与识别抗原和主要组织相容性复合体（MHC）分子的已知T细胞受体（TCR）是由多态性二硫键连接的α链和β链组成的糖蛋白1 - 5。编码这些链的基因与免疫球蛋白基因同源，由在发育过程中重排的V（可变）、J（连接）和C（恒定）区组成6 - 13。TCR在胸腺细胞发育中表达相对较晚，并且仅与称为T3的蛋白质的不变分子复合物相关（参考文献3，14）。未成熟胸腺细胞不表达TCR-T3复合物，但表达编码第三种重排T细胞受体样基因T γ的信使RNA（参考文献13、15 - 20）。在这里，我们报告了一个正常的未成熟T4 − T8 −人胸腺细胞克隆，命名为CII，它不表达T α或T β基因的成熟mRNA，但表达高水平的T γ mRNA。该克隆还表达高水平的表面T3，并且T3的抗体在CII细胞中诱导免疫相关功能。CII表面标记的蛋白质与抗T3的免疫沉淀共沉淀T3分子复合物以及相对分子质量（Mr）为44，000（44K）和62，000（62K）的两种另外的新肽。
The known T-cell receptors (TCRs) involved in the recognition of antigen and major histocompatibility complex (MHC) molecules are glycoproteins comprised of polymorphic disulphide-linkedα-andβ-chains1–5. The genes encoding these chains are homologous to immunoglobulin genes and consist ofV(variable),J(joining) andC(constant) regions that rearrange during development6–13. TCRs are expressed relatively late in thymocyte development and only in association with an invariant molecular complex of proteins termed T3 (refs 3, 14). Immature thymocytes do not express the TCR–T3 complex but do express messenger RNA encoding a third rearranging T-cell receptor-like gene, termed Tγ(refs 13,15–20). Here we report a clone of normal immature T4−T8−human thymocytes, designated CII, which does not express mature mRNA for Tαor Tβgenes, but does express high levels of TγmRNA. This clone also expresses high levels of surface T3, and antibodies to T3 induce immunologically relevant functions in CII cells. Immunoprecipitation of CII surface-labelled proteins with anti-T3 co-precipitates a T3 molecular complex together with two additional and novel peptides of relative molecular mass (Mr) 44,000 (44K) and 62,000 (62K).