Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments
Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments
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DOI：
10.1103/physrevlett.112.088102
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发表时间：
2014-02
影响因子：
8.6
通讯作者：
B. Weinhausen;Oliva Saldanha;R. Wilke;C. Dammann;M. Priebe;M. Burghammer;M. Sprung;S. Köster
中科院分区：
文献类型：
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作者：
B. Weinhausen;Oliva Saldanha;R. Wilke;C. Dammann;M. Priebe;M. Burghammer;M. Sprung;S. Köster
高分辨率X射线成像技术为研究生物细胞的纳米级结构提供了多种可能性。一项具有挑战性的任务仍然是在自然的水环境中用x射线研究细胞。在这里，我们克服了这一限制，提出了扫描X射线衍射测量的光束尺寸在几百纳米的范围内的活的和固定的水合真核细胞在微流体装置，模仿原生环境。固定水合和活细胞之间的直接比较显示出散射信号的明显差异，指向30至50 nm量级的结构变化。
High-resolution x-ray imaging techniques offer a variety of possibilities for studying the nanoscale structure of biological cells. A challenging task remains the study of cells by x rays in their natural, aqueous environment. Here, we overcome this limitation by presenting scanning x-ray diffraction measurements with beam sizes in the range of a few hundred nm on living and fixed-hydrated eukaryotic cells in microfluidic devices which mimic a native environment. The direct comparison between fixed-hydrated and living cells shows distinct differences in the scattering signal, pointing to structural changes on the order of 30 to 50 nm.