DNA-BINDING PROPERTIES AND SECONDARY STRUCTURAL MODEL OF THE HEPATOCYTE NUCLEAR FACTOR-III FORK HEAD DOMAIN
DNA-BINDING PROPERTIES AND SECONDARY STRUCTURAL MODEL OF THE HEPATOCYTE NUCLEAR FACTOR-III FORK HEAD DOMAIN
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DOI：
10.1073/pnas.90.24.11583
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发表时间：
1993-12-15
影响因子：
11.1
通讯作者：
TUCKER, PW
中科院分区：
文献类型：
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作者：
LI, C;TUCKER, PW
QRF-1 [富含谷氨酰胺（Q）的因子1]是一种新克隆的B细胞来源的DNA结合蛋白，其84个氨基酸的片段与肝细胞核因子3/叉头蛋白家族的DNA结合结构域具有显著的序列同源性。在这里，我们证明，这84个氨基酸的结构域是必要的，足够的DNA结合。我们还提出了一个二级结构模型的域。在模型的N-末端部分，基本的钩结构之后是由一个转角分开的两亲性螺旋。两个螺旋内的不变氨基酸残基形成疏水核心。芳香扭结和第三个两亲性螺旋构成结构域的中心。在C末端，两个可变长度的环侧接一个推定的7-氨基酸螺旋，随后是一个短的碱性区域。
An 84-amino acid segment of QRF-1 [glutamine (Q)-rich factor 1], a newly cloned, B-cell-derived DNA-binding protein, shows significant sequence homology with the DNA-binding domains of the hepatocyte nuclear factor 3/fork head family of proteins. Here we demonstrate that this 84-amino acid domain is necessary and sufficient for DNA binding. We also propose a secondary structural model for the domain. At the N-terminal portion of the model, a basic hook structure is followed by two amphipathic helices separated by a turn. Invariant amino acid residues within the two proposed helices form the hydrophobic cores. An aromatic kink and a third amphipathic helix comprise the center of the domain. At the C terminus, two variable-length loops flank a putative 7-amino acid helix followed by a short basic region.