Coherent Motions in Confluent Cell Monolayer Sheets
Coherent Motions in Confluent Cell Monolayer Sheets
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DOI：
10.1016/j.bpj.2014.08.006
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发表时间：
2014-10-07
影响因子：
3.4
通讯作者：
Sun, Sean X.
中科院分区：
文献类型：
--
作者：
Li, Bo;Sun, Sean X.
细胞迁移在许多重要的生理过程中起着关键作用，如胚胎发生、伤口愈合、免疫防御和癌症转移。虽然许多努力已被定向到个别细胞的运动性，集体细胞迁移的机制仍然知之甚少。在这里，我们开发了一个集体运动模型，结合细胞力学和持续的随机运动的个别细胞研究连贯的迁移运动上皮样单层。这个模型，在没有任何外部化学信号的情况下，能够解释从两个粘附细胞到多细胞组装体的系统中所见的坐标旋转运动。我们发现，主动持久力和随机偏振涨落之间的竞争是鲁棒旋转的原因。细胞之间的被动机械耦合是必要的，但细胞之间的主动化学信号传导不是必要的。预测的角运动还取决于底层基板的几何形状：细胞表现出集体旋转圆形基板上，但显示线性的来回运动的长和窄的基板上。
Cell migration plays a pivotal role in many physiologically important processes such as embryogenesis, wound-healing, immune defense, and cancer metastasis. Although much effort has been directed toward motility of individual cells, the mechanisms underpinning collective cell migration remain poorly understood. Here we develop a collective motility model that incorporates cell mechanics and persistent random motions of individual cells to study coherent migratory motions in epithelial-like monolayers. This model, in absence of any external chemical signals, is able to explain coordinate rotational motion seen in systems ranging from two adherent cells to multicellular assemblies. We show that the competition between the active persistent force and random polarization fluctuation is responsible for the robust rotation. Passive mechanical coupling between cells is necessary but active chemical signaling between cells is not. The predicted angular motions also depend on the geometrical shape of the underlying substrate: cells exhibit collective rotation on circular substrates, but display linear back-and-forth motion on long and narrow substrates.