Counting single chromophore molecules for ultrasensitive analysis and separations on microchip devices
Counting single chromophore molecules for ultrasensitive analysis and separations on microchip devices
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DOI：
10.1021/ac9707242
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发表时间：
1998-02-01
影响因子：
7.4
通讯作者：
Ramsey, JM
中科院分区：
文献类型：
--
作者：
Fister, JC;Jacobson, SC;Ramsey, JM
在微机械电泳道中进行的15 PM罗丹明6G和30 PM罗丹明B的分离是通过计数单个分子的荧光脉冲来检测的。根据被检测分子的数量和迁移时间分布估计了迁移时间、峰宽和分析物浓度。在>；99%置信度下估计的浓度检测限为1.7 pm罗丹明6G和8.5 pm罗丹明B。
Separations of 15 pM rhodamine 6G and 30 pM rhodamine B performed in a micromachined electrophoresis channel were detected by counting fluorescence bursts from individual molecules. The migration times, peak widths, and analyte concentrations were estimated from the number and the migration time distribution of the detected molecules. Concentration detection limits estimated at >99% confidence were 1.7 pM rhodamine 6G and 8.5 pM rhodamine B. The separations required