Comparison of the expression and activity of the lipogenic pathway in human and rat adipose tissue
Comparison of the expression and activity of the lipogenic pathway in human and rat adipose tissue
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DOI：
10.1194/jlr.m300235-jlr200
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发表时间：
2003-11-01
影响因子：
6.5
通讯作者：
Beylot, M
中科院分区：
文献类型：
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作者：
Letexier, D;Pinteur, C;Beylot, M
认为人体脂肪生成活性低于大鼠脂肪组织。这可以通过不同的营养条件来解释，即大鼠的高碳水化合物（HCHO）饮食和人类的高脂肪（HF）饮食。在接受HCHO或BY饮食的大鼠和人类、自由进食的人类和肥胖受试者中对脂肪组织进行采样（吸收后状态）。我们测量了1）脂肪酸合成酶（FAS）、乙酰辅酶A羧化酶1（ACC 1）、固醇调节元件结合蛋白1c（SREBP-1c）和碳水化合物反应元件结合蛋白（ChREBP）的mRNA浓度，2）SREBP-1c蛋白，3）FAS活性。FAS、ACC 1、ChREBP和SREBP 1-c mRNA浓度不受人类或大鼠饮食的影响。FAS和ACC 1 mRNA在人的表达低于大鼠（P < 0.05）。FAS活性不受饮食的影响，并且在人类中较低（P < 0.05）。SREBP-1c mRNA在大鼠和人中的浓度相似，但SREBP-1c蛋白的前体和成熟形式在人中的丰度较低（P < 0.05）。ChREBP mRNA浓度在人类中低于大鼠。jlr总之，脂肪组织的脂肪生成能力在人类中低于大鼠。这与饮食差异无关，可能是由于SREBP-1c蛋白丰度较低。ChREBP的表达减少也可能发挥作用。Letexier，D.，C. Pinteur，V. Large，V Frring和M.贝伊洛人和大鼠脂肪组织中脂肪生成途径的表达和活性的比较。
Lipogenesis is considered less active in human than in rat adipose tissue. This could be explained by different nutritional conditions, namely high-carbohydrate (HCHO) diet in rats and high-fat (HF) diet in humans. Adipose tissue was sampled (postabsorptive state) in rats and humans receiving HCHO or BY diets, ad libitum fed humans, and obese subjects. We measured 1) mRNA concentrations of fatty acid synthase (FAS), acetyl-CoA carboxylase 1 (ACC1), sterol regulatory element binding protein 1c (SREBP-1c), and carbohydrate response element binding protein (ChREBP), 2) SREBP-1c protein, and 3) FAS activity. FAS, ACC1, ChREBP, and SREBP1-c mRNA concentrations were unaffected by diet in humans or in rats. FAS and ACC1 mRNA levels were lower in humans than in rats (P < 0.05). FAS activity was unaffected by diet and was lower in humans (P < 0.05). SREBP-1c mRNA concentrations were similar in rats and humans, but the precursor and mature forms of SREBP-1c protein were less abundant in humans (P < 0.05). ChREBP mRNA concentrations were lower in humans than in rats.jlr In conclusion, the lipogenic capacity of adipose tissue is lower in humans than in rats. This is not related to differences in diet and is probably explained by lower abundance of SREBP-1c protein. A decreased expression of ChREBP could also play a role.-Letexier, D., C. Pinteur, V. Large, V Frering, and M. Beylot. Comparison of the expression and activity of the lipogenic pathway in human and rat adipose tissue..