Aptamer-Based Multicolor Fluorescent Gold Nanoprobes for Multiplex Detection in Homogeneous Solution
Aptamer-Based Multicolor Fluorescent Gold Nanoprobes for Multiplex Detection in Homogeneous Solution
复制标题
基于适体的多色荧光金纳米探针用于均质溶液中的多重检测
DOI：
10.1002/smll.200901012
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发表时间：
2010-01-18
期刊：
影响因子：
13.3
通讯作者：
Fan, Chunhai
中科院分区：
文献类型：
--
作者：
Zhang, Juan;Wang, Lihua;Fan, Chunhai
报道了一种用于小分子目标均相检测的新型双荧光金纳米探针的设计，其结合了适配体的特异性结合能力和金纳米颗粒（AuNPs）的荧光猝灭能力。染料标记的适体及其具有硫醇标记的互补序列在AuNP的表面共组装。作为概念证明，它表明，这样的荧光金纳米探针可以同时检测腺苷，钾离子，可卡因具有高选择性。这种潜在的通用策略被证明是有前途的小分子靶点的快速筛选。
The design of a novel multicolor fluorescent gold nanoprobe for homogeneous detection of small‐molecule targets is reported, which combines the specific binding abilities of aptamers with the ultrahigh quenching ability of gold nanoparticles (AuNPs). Dye‐tagged aptamers and their complementary sequence with thiol labels are co‐assembled at the surface of AuNPs. As a proof of concept, it is demonstrated that such a multicolor fluorescent gold nanoprobe can simultaneously detect adenosine, potassium ion, and cocaine with high selectivity. This potentially generic strategy is shown to be promising for rapid screening of small molecular targets.