Palmitate promotes the paracrine effects of macrophages on vascular smooth muscle cells: the role of bone morphogenetic proteins.
Palmitate promotes the paracrine effects of macrophages on vascular smooth muscle cells: the role of bone morphogenetic proteins.
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DOI：
10.1371/journal.pone.0029100
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lee SH
中科院分区：
文献类型：
--
作者：
Chung JH;Jeon HJ;Hong SY;Lee DL;Lee KH;Kim SH;Han YS;Manabe I;Miller YI;Lee SH
已知饱和脂肪酸可激活巨噬细胞并诱导血管炎症。虽然活化的巨噬细胞的细胞因子影响其他血管细胞，但饱和脂肪酸对巨噬细胞旁分泌效应的影响尚未完全了解。在这里，我们研究了棕榈酸对巨噬细胞对血管平滑肌细胞（SMC）及其介质的影响。用棕榈酸刺激的RAW264.7细胞的条件培养基处理后，SMC增殖显著增加。SMC迁移被认为是更大的棕榈酸条件培养基治疗后。棕榈酸条件培养基处理的SMC中SM α-肌动蛋白和SM 22 α减少。当用棕榈酸刺激时，RAW264.7细胞向细胞培养基中分泌更多的骨形态发生蛋白（BMP）2和BMP 4。SMC增殖，迁移和表型变化减弱后，治疗的中和抗体对骨形成蛋白或敲低骨形成蛋白的siRNA。通过重组BMP 2和BMP 4对SMC的直接处理进一步证实了这些蛋白的影响。尤其是BMPs对SMC迁移表型改变的影响明显，而对SMC增殖的影响似乎不明显或不明显。总之，棕榈酸促进巨噬细胞对SMC增殖、迁移和表型改变的旁分泌作用。刺激的巨噬细胞的作用至少部分由BMP 2和BMP 4介导。这些结果表明，一种新的机制连接饱和脂肪酸和血管疾病的进展，可能是由骨形成蛋白介导的巨噬细胞。
Saturated fatty acids are known to activate macrophages and induce vascular inflammation. Although cytokines from activated macrophage influence other vascular cells, the influence of saturated fatty acids on the paracrine effect of macrophages is not fully understood yet. Here we examined the impact of palmitate on the effect of macrophages on vascular smooth muscle cells (SMCs) and their mediators. SMCs proliferation increased significantly after treatment with conditioned media from palmitate-stimulated RAW264.7 cells. SMC migration was found to be greater after treatment with palmitate-conditioned media. SM α-actin and SM22α were decreased in SMCs treated with palmitate-conditioned media. When stimulated with palmitate, RAW264.7 cells secreted more bone morphogenetic protein (BMP)2 and BMP4 into the cell culture media. SMC proliferation, migration, and phenotypic changes were attenuated after treatment of neutralizing antibodies against BMPs or knockdown of BMPs with siRNA. The influences of these proteins were further confirmed by direct treatment of recombinant BMP2 and BMP4 on SMCs. Particularly, the effects of BMPs on SMC migration on phenotypic change were obvious, whereas their effect on SMC proliferation seemed not significant or modest. In conclusion, palmitate promoted macrophages' paracrine effects on SMC proliferation, migration, and phenotypic change. The effect of stimulated macrophages was mediated, at least in part, by BMP2 and BMP4. These results suggest a novel mechanism linking saturated fatty acids and the progression of vascular diseases that is possibly mediated by BMPs from macrophages.
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影响因子：
4.8
作者：
Weigert, C;Brodbeck, K;Schleicher, ED
通讯作者：
Schleicher, ED
DOI：
10.1073/pnas.0510973103
发表时间：
2006-02-21
影响因子：
11.1
作者：
Chen, CN;Li, YSJ;Chiu, JJ
通讯作者：
Chiu, JJ
影响因子：
2.2
作者：
Corriere, Matthew A.;Rogers, Chris M.;Guzman, Raul J.
通讯作者：
Guzman, Raul J.
影响因子：
5.3
作者：
Hayashi, Ken'ichiro;Nakamura, Seiji;Sobue, Kenji
通讯作者：
Sobue, Kenji
影响因子：
4.8
作者：
Lee, JY;Sohn, KH;Hwang, D
通讯作者：
Hwang, D