Temporal surge of glycosyltransferase activities in the genital tract of the hamster during the estrous cycle.
Temporal surge of glycosyltransferase activities in the genital tract of the hamster during the estrous cycle.
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发情周期期间仓鼠生殖道糖基转移酶活性的暂时激增。
DOI：
10.1095/biolreprod54.5.1032
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发表时间：
1996
影响因子：
3.6
通讯作者：
Araki,Y
中科院分区：
文献类型：
--
作者：
Tulsiani,DR;Chayko,CA;Orgebin-Crist,MC;Araki,Y
哺乳动物精子在交配和受精之间必须经历成熟的变化。这些生化和功能的改变，统称为获能，发生在精子穿越雌性生殖道。准备性生化变化包括精子表面分子的去除、修饰和重组。虽然所有的变化的细节是未知的，凝集素结合的研究提供了证据表明，精子表面糖蛋白的碳水化合物部分在获能过程中被修改。为了深入了解精子质膜糖蛋白的潜在修饰，我们在发情周期的4天内定量测定了仓鼠子宫和输卵管液中糖蛋白修饰酶的活性。已知这些酶通过添加糖残基（糖基转移酶）或通过去除末端糖残基（糖苷酶）来修饰现有的糖蛋白。这些研究的数据表明：1）测定的所有糖基转移酶活性水平（唾液酸转移酶、岩藻糖基转移酶、半乳糖基转移酶和N-乙酰氨基葡萄糖基转移酶）在排卵开始时（第1天）子宫液中可忽略不计，但在排卵前（第4天）急剧增加;（2）在排卵开始时（第1天），输卵管液中四种糖基转移酶的活性水平较高然后在发情周期的剩余时间里逐渐减少（第2天至第4天）; 3）所有糖水解酶活性的水平（酸性α-D-甘露糖苷酶、β-D-半乳糖苷酶、β-D-葡萄糖醛酸酶、β-D-氨基葡萄糖苷酶，和α-L-岩藻糖苷酶）和蛋白质在子宫和输卵管液中在4天的周期内没有广泛的变化。这些结果表明仓鼠生殖道液中糖基转移酶活性的时间激增。糖蛋白修饰酶的时间变化可能对受精部位精子质膜和透明质酸糖蛋白的糖基化产生影响，或可能在着床前改变子宫内受精卵的表面糖蛋白。
Mammalian spermatozoa must undergo maturational changes between the events of mating and fertilization. These biochemical and functional alterations, collectively termed capacitation, take place as spermatozoa traverse the female reproductive tract. The preparatory biochemical changes include removal, modification, and reorganization of sperm surface molecules. Although details of all the changes are not known, lectin binding studies have provided evidence suggesting that carbohydrate moieties of sperm surface glycoproteins are modified during capacitation. In an attempt to gain insight into the potential modifications of sperm plasma membrane glycoproteins, we quantified glycoprotein-modifying enzyme activities in the uterine and oviductal fluid of the hamster during the 4 days of the estrous cycle. These enzymes are known to modify existing glycoproteins, either by adding sugar residues (glycosyltransferases) or by removing terminal sugar residues (glycosidases). Data from these studies showed that 1) levels of all glycosyltransferase activities assayed (sialyltransferase, fucosyltransferase, galactosyltransferase, andN-acetylglucosaminyltransferase) were negligible in the uterine fluid at the onset of ovulation (Day 1) but sharply increased preceding ovulation (Day 4); 2) levels of the four glycosyltransferase activities assayed were higher in the oviductal fluid at the onset of ovulation (Day 1) and then gradually decreased through the remainder of the estrous cycle (Day 2 to Day 4); 3) levels of all glycohydrolase activities (acidic α-D-mannosidase, β-D-galactosidase, β-D-glucuronidase, β-D-glucosaminidase, and α-L-fucosidase) and protein in the uterine and oviductal fluids did not vary widely during the 4 days of the cycle. These results demonstrate a temporal surge of glycosyltransferase activities in the genital tract fluids of the hamster. The temporal changes in the glycoprotein-modifying enzymes may have an effect on the glycosylation of sperm plasma membrane and zona pellucida glycoproteins at the site of fertilization or may alter the surface glycoproteins of the fertilized egg in the uterus prior to implantation.