hMEF2C gene encodes skeletal muscle- and brain-specific transcription factors
hMEF2C gene encodes skeletal muscle- and brain-specific transcription factors
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DOI：
10.1128/mcb.13.4.2564-2577.1993
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发表时间：
1993-04
影响因子：
5.3
通讯作者：
John C. McDERMOTf;M. C. Cardoso;YU YIE-TEH;T. V. Andres;Dana Leifer;Dimitri Krainc;Stuart A. Lipton;Bernardo Nadal GINARDl;V. Mahdavi
中科院分区：
文献类型：
--
作者：
John C. McDERMOTf;M. C. Cardoso;YU YIE-TEH;T. V. Andres;Dana Leifer;Dimitri Krainc;Stuart A. Lipton;Bernardo Nadal GINARDl;V. Mahdavi
肌细胞增强结合因子2 （MEF2）位点是许多肌肉特异性增强因子和启动因子的重要组成部分，这些增强因子和启动因子结合来自肌肉和大脑的核蛋白。最近，我们克隆了一个由两个基因产生的MEF2转录因子家族，这两个基因在mRNA水平上广泛表达，并通过转录后过程产生组织特异性亚型（y - t）。于，R. E. Breitbart， L. B. Smoot， Y. Lee， V. Mahdavi， B. Nadal-Ginard，基因发展，6:1783-1798,1992)。在这里，我们报道了编码四个MEF2因子的cDNA克隆的分离和功能表征，这些因子来源于一个我们命名为hMEF2C的独立基因。与先前报道的基因相比，hMEF2C基因的转录本仅限于骨骼肌和大脑。其中一个外显子只存在于大脑转录本中。该基因的产物具有dna结合和反式激活活性，与先前报道的MEF2因子没有区别。hMEF2C基因在肌源性分化后期被诱导，其表达仅限于皮质神经元的一个子集。这种转录因子在神经元子集中的潜在靶标目前尚不清楚。与其他MEF2基因的普遍表达相比，hMEF2C严格的组织特异性表达模式表明，hMEF2C因子在肌肉和神经发生中具有不同的调节模式和潜在的重要作用。
The myocyte enhancer-binding factor 2 (MEF2) site is an essential element of many muscle-specific enhancers and promoters that binds nuclear proteins from muscle and brain. Recently, we have cloned a family of MEF2 transcription factors produced by two genes that, at the mRNA level, are broadly expressed and produce tissue-specific isoforms by posttranscriptional processes (Y.-T. Yu, R. E. Breitbart, L. B. Smoot, Y. Lee, V. Mahdavi, and B. Nadal-Ginard, Genes Dev. 6:1783-1798, 1992). Here, we report the isolation and functional characterization of cDNA clones encoding four MEF2 factors derived from a separate gene that we have named hMEF2C. In contrast to those of the previously reported genes, the transcripts of the hMEF2C gene are restricted to skeletal muscle and brain. One of the alternate exons is exclusively present in brain transcripts. The products of this gene have DNA-binding and trans-activating activities indistinguishable from those of the previously reported MEF2 factors. The hMEF2C gene is induced late during myogenic differentiation, and its expression is limited to a subset of cortical neurons. The potential targets for this transcription factor in a subset of neurons are not known at this time. The strict tissue-specific pattern of expression of hMEF2C in comparison with the more ubiquitous expression of other MEF2 genes suggests a different mode of regulation and a potentially important role of hMEF2C factors in myogenesis and neurogenesis.