Rapid multisample separation of the five most widespread animal glycosaminoglycans.
Rapid multisample separation of the five most widespread animal glycosaminoglycans.
复制标题
五种最常见的动物糖胺聚糖的快速多样品分离。
DOI：
10.1016/s0003-2697(79)80113-x
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发表时间：
1979
影响因子：
2.9
通讯作者：
V. Chiarugi
中科院分区：
文献类型：
--
作者：
R. Cappelletti;M. Del Rosso;V. Chiarugi
从对各种生物系统的研究中出现的最常见的图片，包括正常和转化的细胞系（1，2），细胞间基质（3），组织细胞（4）等，显示了五种分子类型的糖胺聚糖（GAG）;透明质酸（HA）、硫酸乙酰肝素（HS）、硫酸皮肤素（DS）、4-硫酸软骨素（C4 S）和6-硫酸软骨素（C6 S）。[2]在解析这个异质分子家族的组分时遇到了许多困难。目前，需要多种技术的组合来识别单个糖胺聚糖：不允许直接比较的二维电泳（5）、离子交换色谱法以及酶降解或化学分解以识别水解产物（6）。我们在这里描述了一种非常快速、廉价和高度可重复的技术，与其他电泳方法相比，该技术具有以下优点：（10-30分钟）;高灵敏度（每种化合物0.1/xg）;提高了生物样本中最常描述的所有五种化合物的分辨率;纸张的可管理性更好，无需
The most frequent picture emerging from the studies on a variety of biological systems including normal and transformed cell lines (1, 2), intercellular matrix (3), tissular cells (4), etc., shows five molecular classes of glycosaminoglycans (GAGs); hyaluronic acid (HA), heparan sulfate (HS), dermatan sulfate (DS), chondroitin-4-sulfate (C4S), and chondroitin-6-sulfate (C6S). 2 Many difficulties have been encountered in the resolution of the components of this heterogeneous family of molecules. Presently a combination of various techniques is required in order to identify single GAGs: two-dimensional electrophoresis (5) which does not allow direct comparison, ion-exchange chromatography, and enzyme degradation or chemical breakdown with identification of the hydrolysis products (6). We describe here a very rapid, inexpensive, and highly reproducible technique which, compared with other electrophoretic procedures, exhibits the following advantages: possibility to compare directly up to eight samples in a single run; very short migration time (10-30 min); high sensitivity (0.1/xg per compound); improved resolution of all the five compounds most frequently described in biological samples; a greater manageability of the sheet and no need to