ENCAPSULATION OF MACROMOLECULES BY LIPID VESICLES UNDER SIMULATED PREBIOTIC CONDITIONS
ENCAPSULATION OF MACROMOLECULES BY LIPID VESICLES UNDER SIMULATED PREBIOTIC CONDITIONS
复制标题
DOI：
10.1007/bf01733047
复制
发表时间：
1982-01-01
影响因子：
3.9
通讯作者：
BARCHFELD, GL
中科院分区：
文献类型：
--
作者：
DEAMER, DW;BARCHFELD, GL
磷脂囊泡（脂质体）进行脱水-水化循环的存在下，6-羧基荧光素或鲑鱼精子DNA。我们发现，囊泡融合成多层结构，在脱水与溶质截留在层之间。在再水化时，薄片膨胀并形成含有溶质的大泡状结构。该模型可用于研究在益生元条件下脂质膜对大分子的包封以形成原细胞结构。
Phospholipid vesicles (liposomes) were subjected to dehydration-hydration cycles in the presence of 6-carboxyfluorescein or salmon sperm DNA. We found that the vesicles fused into multilamellar structures during dehydration with solutes trapped between the lamellae. Upon rehydration the lamellae swelled and formed large vesicular structures containing solute. This model can be used to study encapsulation of macromolecules by lipid membranes to form protocellular structures under prebiotic conditions.