Comparative analysis of PvPAP gene family and their functions in response to phosphorus deficiency in common bean.
Comparative analysis of PvPAP gene family and their functions in response to phosphorus deficiency in common bean.
复制标题
菜豆PvPAP基因家族及其响应缺磷功能的比较分析
DOI：
10.1371/journal.pone.0038106
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tian J
中科院分区：
文献类型：
--
作者：
Liang C;Sun L;Yao Z;Liao H;Tian J
研究背景紫色酸性磷酸酶（PAPs）在植物对缺磷的适应中起着重要作用。然而，它们在菜豆磷效率中的作用是不完整的。主要发现五个PvPAPs分离和测序普通豆类。系统发育分析表明，PvPAPs可分为两大类，一类是小分子量的PvPAPs，另一类是大分子量的PvPAPs。其中，PvPAP 3、PvPAP 4和PvPAP 5属于小群体，而另外两个属于大群体。35 S：PvPAPs-GFP在洋葱表皮细胞上的瞬时表达证实了PvPAPs的亚细胞定位差异，表明PvPAPs在菜豆中具有功能性。定量PCR结果表明，大多数PvPAPs被磷酸盐（Pi）饥饿上调。其中，小群体PvPAPs的表达对磷饥饿的反应更强，尤其是在磷高效基因型G19833的根部。然而，只有过量表达PvPAP 1和PvPAP 3才能显著提高转基因菜豆毛状根对胞外dNTPs的利用率。此外，在拟南芥中过表达PvPAP 3增强植物生长和总P含量时，dNTPs作为唯一的外部P源供应。结论菜豆中的PvPAPs在蛋白质结构、对缺磷的反应和亚细胞定位方面存在差异。其中PvPAP 1和PvPAP 3可能都是利用胞外dNTPs的。
Background Purple acid phosphatases (PAPs) play a vital role in adaptive strategies of plants to phosphorus (P) deficiency. However, their functions in relation to P efficiency are fragmentary in common bean. Principal Findings Five PvPAPs were isolated and sequenced in common bean. Phylogenetic analysis showed that PvPAPs could be classified into two groups, including a small group with low molecular mass, and a large group with high molecular mass. Among them, PvPAP3, PvPAP4 and PvPAP5 belong to the small group, while the other two belong to the large group. Transient expression of 35S:PvPAPs-GFP on onion epidermal cells verified the variations of subcellular localization among PvPAPs, suggesting functional diversities of PvPAPs in common bean. Quantitative PCR results showed that most PvPAPs were up-regulated by phosphate (Pi) starvation. Among them, the expression of the small group PvPAPs responded more to Pi starvation, especially in the roots of G19833, the P-efficient genotype. However, only overexpressing PvPAP1 and PvPAP3 could result in significantly increased utilization of extracellular dNTPs in the transgenic bean hairy roots. Furthermore, overexpressing PvPAP3 in Arabidopsis enhanced both plant growth and total P content when dNTPs were supplied as the sole external P source. Conclusions The results suggest that PvPAPs in bean varied in protein structure, response to P deficiency and subcellular localization. Among them, both PvPAP1 and PvPAP3 might function as utilization of extracellular dNTPs.
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DOI：
10.1016/s0167-4838(97)00055-1
发表时间：
1997-08-15
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
影响因子：
--
作者：
Olczak, M;Watorek, W;Morawiecka, B
通讯作者：
Morawiecka, B
影响因子：
7.4
作者：
Liang, Cuiyue;Tian, Jiang;Liao, Hong
通讯作者：
Liao, Hong
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
7.2
作者：
del Pozo, JC;Allona, I;Paz-Ares, J
通讯作者：
Paz-Ares, J
影响因子：
3
作者：
Fan, MS;Zhu, JM;Lynch, JP
通讯作者：
Lynch, JP