The raspberry bushy dwarf virus 1b gene enables pollen grains to function efficiently in horizontal pollen transmission.
The raspberry bushy dwarf virus 1b gene enables pollen grains to function efficiently in horizontal pollen transmission.
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覆盆子丛生矮化病毒 1b 基因使花粉粒能够在花粉水平传播中有效发挥作用。
DOI：
10.1016/j.virol.2020.01.005
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Yoshikawa Nobuyuki
中科院分区：
文献类型：
--
作者：
Masamichi Isogai;Takanori Matsudaira;Miyoshi Kotaro;Shimura Takuya;Yoshikawa Nobuyuki
与野生型树莓丛矮病毒相比，毒力缺陷的树莓丛矮病毒1b缺失突变体(RbΔ1bStop)的水平传粉能力显著降低。我们对RbΔ1bStop感染植株的花粉中病毒基因组(G)RNA的积累进行了评估，发现花粉中的病毒gRNA比感染wtRBDV的植株的要少。此外，RbΔ1bStop感染的1b转基因植株(1b-植株)的花粉在授粉后向健康植株水平传播病毒的效率高于RbΔ1bStop感染的野生型植株的花粉。此外，RbΔ1bStop侵染1b植株的花粉中病毒gRNA的积累量高于RbΔ1bStop感染野生型植株的花粉。我们认为1b增加了从延长的花粉粒中释放的病毒gRNAs的数量。
Horizontal pollen transmission by the raspberry bushy dwarf virus 1b deletion mutant (RBΔ1bstop), which is defective in virus virulence, was significantly decreased compared to wild-type raspberry bushy dwarf virus (wtRBDV). We assessed accumulation of viral genomic (g) RNAs in pollen grains from RBΔ1bstop-infected plants and found that the pollen grains had less viral gRNA than those from wtRBDV-infected plants. In addition, pollen grains from 1b-expressing transgenic plants (1b-plants) infected with RBΔ1bstop were more efficient in horizontal virus transmission to healthy plants after pollination than pollen from RBΔ1bstop-infected wild type plants. Moreover, viral gRNA accumulation in pollen grains from RBΔ1bstop-infected 1b-plants was higher than in pollen from RBΔ1bstop-infected wild type plants. We suggest that 1b increases the amount of viral gRNAs released from elongating pollen grains.