Oct4, a Novel Marker for Human Gastric Cancer
Oct4, a Novel Marker for Human Gastric Cancer
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DOI：
10.1002/jso.21270
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发表时间：
2009-06-01
影响因子：
2.5
通讯作者：
Xu, Xue-Jing
中科院分区：
文献类型：
--
作者：
Chen, Zhong;Xu, Wen-Rong;Xu, Xue-Jing
背景和目的：八聚体-4（Oct 4）是一种参与调节人胚胎干细胞（ESCs）的转录因子，可能在肿瘤发生中发挥作用。方法：收集62例胃癌患者的原发癌组织和癌旁组织，采用逆转录-PCR（RT-PCR）和实时荧光定量PCR（real-time PCR）方法检测胃癌组织中Oct 4的表达。对照组为萎缩性胃炎和胃溃疡各20例。采用Western blotting和免疫组化方法检测胃癌组织和癌旁组织中Oct 4蛋白的表达。结果：胃癌组织中Oct 4的表达水平明显高于癌旁组织、萎缩性胃炎和胃溃疡组织，胃癌组织中Oct 4的表达水平明显高于癌旁组织、萎缩性胃炎和胃溃疡组织。另外。胃癌中Oct 4的表达与其分化程度有关。但与患者年龄、性别、肿瘤大小、TNM分期无关。结论：Oct 4可能是胃癌发生、发展的潜在生物标志物。和人GC的分化。外科肿瘤学杂志2009;99：414-419。(C)2009 Wiley-Liss，Inc.
Background and Objective: Octamer-4 (Oct4), a transcription factor involved in regulating human embryonic stern cells (ESCs), may play a role in tumorigenesis. Since little is known about the efficacy of Oct4 its a potential biomarker for gastric cancer (GC), we investigated its expression in GC tissues and its relationship to various clinicopathological parameters.Methods: Primary tumor tissues and matching, adjacent non-cancerous tissues were obtained from 62 GC patients, and Oct4 expression was examined by reverse transcription-PCR (RT-PCR) and real-time PCR. Twenty biopsy specimens of atrophic gastritis and gastric ulcer individually were collected as control. To detect Oct4 expression in the paired GC and non-cancerous tissues at the protein level, Western blotting and immunohistochemistry (IHC) were employed. Correlation analyses were conducted to assess the relationship between Oct4 expression and clinicopathological parameters.Results: Oct4 expression levels were higher in GC tissues compared to matching, adjacent non-cancerous tissues, atrophic gastritis and gastric ulcer tissues. Additionally. Oct4 expression in GC tumors correlated with their differentiation status. but not with patient age or gender, tumor size, TNM stage. depth of invasion, Or the presence of lymph node metastasis.Conclusions: Oct4 may be a potential biomarker for the initiation, progression. and differentiation of human GC. J. Surg. Oncol. 2009;99:414-419. (C) 2009 Wiley-Liss, Inc.