PHOSPHORYLATION OF THE CALCIUM-ANTAGONIST RECEPTOR OF THE VOLTAGE-SENSITIVE CALCIUM-CHANNEL BY CAMP-DEPENDENT PROTEIN-KINASE
PHOSPHORYLATION OF THE CALCIUM-ANTAGONIST RECEPTOR OF THE VOLTAGE-SENSITIVE CALCIUM-CHANNEL BY CAMP-DEPENDENT PROTEIN-KINASE
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DOI：
10.1073/pnas.82.8.2528
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
CATTERALL, WA
中科院分区：
文献类型：
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作者：
CURTIS, BM;CATTERALL, WA
电压敏感性Ca通道受cAMP和蛋白质磷酸化调节。来自横小管膜的电压敏感性Ca通道的Ca拮抗剂受体[从兔快肌纯化]由3个亚基组成，命名为α，β。和γ。cAMP依赖性蛋白激酶的催化亚基磷酸化α-腺苷酸和α-腺苷酸。和β亚基的纯化受体的速率和程度，表明他们是这种酶的潜在生理底物。α-磷酸化和β通过二维凝胶电泳分析横小管膜中的亚基。在完整的横小管膜中，α。亚基未被显着磷酸化。的β通过分子量、等电点和与麦胚凝集素-琼脂糖凝胶的结合，鉴定出该亚基是横小管膜上cAMP依赖性蛋白激酶选择性磷酸化的底物之一。cAMP依赖性磷酸化的β钙拮抗剂受体亚基的改变可能是钙通道功能的重要调节机制。
Voltage-sensitive Ca channels are regulated by cAMP and protein phosphorylation. The Ca antagonist receptor of the voltage-sensitive Ca channel from transverse-tubule membranes [purified from rabbit fast muscle] consists of 3 subunits, designated .alpha., .beta. and .gamma.. The catalytic subunit of cAMP-dependent protein kinase phosphorylates both the .alpha. and .beta. subunits of the purified receptor at a rate and extent that suggests they are potential physiological substrates of this enzyme. The phosphorylation of the .alpha. and .beta. subunits in transverse-tubule membranes was analyzed by 2-dimensional gel electrophoresis. In intact transverse-tubule membranes, the .alpha. subunit is not significantly phosphorylated. The .beta. subunit, identified by its MW, pI and binding to wheat germ agglutinin-Sepharose, was 1 of the substrates selectively phosphorylated by cAMP-dependent protein kinase in transverse-tubule membranes. cAMP-dependent phosphorylation of the .beta. subunit of the Ca antagonist receptor may be an important regulatory mechanism for Ca channel function.