Fabrication of continuous apatite-graded collagen sponges via electrolysis method
Fabrication of continuous apatite-graded collagen sponges via electrolysis method
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DOI：
10.1039/c9tb00791a
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发表时间：
2019-07-07
影响因子：
7
通讯作者：
Ikoma, Toshiyuki
中科院分区：
文献类型：
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作者：
Irawan, Vincent;Sasaki, Yusuke;Ikoma, Toshiyuki
已经研究了用于骨软骨缺损愈合的磷灰石和胶原的多层支架，以通过不同的层优化再生线索;然而，多层支架在植入宿主体内后表现出分层。在这项研究中，作为一种替代多层支架，胶原海绵与磷灰石含量梯度制造通过电解法。在i = 22 A m（-2）的恒定电流模式下，以2、3或4cm的不同电极距离对pH 3.0的酸性溶液进行电解，所述酸性溶液包括0.5wt%的胶原和0.1wt%的羟基磷灰石。在电解过程中，高pH值的口袋和胶原蛋白水凝胶同时形成在阴极附近，而低pH值的口袋附近形成的阳极，由于产生的羟基和氢离子，分别。水凝胶的厚度逐渐增长到约三分之一的电极距离。随后，将水凝胶冷冻干燥以形成海绵。低结晶磷灰石沉淀，特别是，最接近阴极的水凝胶区域比离阴极最远的区域更矿化。最靠近阴极的海绵区域的磷灰石/胶原蛋白比为0.34 +/- 0.04，随着与阴极的距离逐渐降低至0.22 +/- 0.04、0.20 +/- 0.05和0.14 +/- 0.02。胶原蛋白的生物相容性没有改变的电解处理，证明了前成骨细胞在电解海绵的附着和增殖。总之，电解法是一个强大的工具，用于制造胶原海绵与连续梯度磷灰石组成。
Multi-layered scaffolds of apatite and collagen for the healing of osteochondral defects have been investigated for optimized regenerative cues by distinct layers; however, multi-layered scaffolds exhibit delamination after implantation in the host body. In this study, as an alternative to multi-layered scaffolds, collagen sponges with an apatite content gradient were fabricated via an electrolytic method. Electrolysis at a constant current mode of i = 22 A m(-2) with different electrode distances of 2, 3, or 4 cm was conducted for an acidic solution at pH 3.0, including collagen at 0.5 wt% and hydroxyapatite at 0.1 wt%. During electrolysis, a high pH pocket and collagen hydrogel were concurrently formed in the vicinity of the cathode, whereas a low pH pocket was formed near the anode due to the generation of hydroxyl and hydrogen ions, respectively. The thickness of the hydrogels gradually grew to approximately one-third of the electrode distance. Subsequently, the hydrogels were freeze-dried to form sponges. Low-crystalline apatite was precipitated, and particularly, the hydrogel region closest to the cathode was more mineralized than the region farthest from the cathode. The sponge region closest to the cathode exhibited a ratio of apatite/collagen of 0.34 +/- 0.04, which gradually decreased with the distance from cathode to 0.22 +/- 0.04, 0.20 +/- 0.05, and 0.14 +/- 0.02. The biocompatibility of collagen was unaltered by the electrolysis treatment, as evidenced by the attachment and proliferation of pre-osteoblastic cells in the electrolyzed sponges. In conclusion, the electrolysis method is a powerful tool for the fabrication of collagen sponges with a continuous graded apatite composition.