Bright monomeric red fluorescent protein with an extended fluorescence lifetime
Bright monomeric red fluorescent protein with an extended fluorescence lifetime
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DOI：
10.1038/nmeth1062
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发表时间：
2007-07-01
期刊：
影响因子：
48
通讯作者：
Chudakov, Dmitriy M.
中科院分区：
文献类型：
--
作者：
Merzlyak, Ekaterina M.;Goedhart, Joachim;Chudakov, Dmitriy M.
荧光蛋白已经成为非常流行的体内成像工具，特别是用于研究活细胞中蛋白质的定位、运动和相互作用。TagRFP是一种红色荧光蛋白单体，具有亮度高、发色团成熟完全、荧光寿命长、ph稳定性高等特点。这些特性使TagRFP成为蛋白质定位研究和荧光共振能量转移（FRET）应用的优秀标签。
Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.